Evidence map›Paper›PMID 40390102›Full record

ArticleJournal of animal science and biotechnology2025

The comprehensive transcriptomic atlas of porcine immune tissues and the peripheral blood mononuclear cell (PBMC) immune dynamics reveal core immune genes.

Qingyao Zhao, Jiahao Wang, Fuping Ma, Quanzhen Chen, Huatao Liu, Jinyan Yang, Siqian Chen, Yongjie Tang, Siyuan Mi, Lulu Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Qingyao ZhaoState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Jiahao WangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Fuping MaState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Quanzhen ChenState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Huatao LiuState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Jinyan YangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Siqian ChenState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Yongjie TangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Siyuan MiState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Lulu WangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Xini WangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Guohong LiuState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Kai XingState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China.
Ying YuState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China. yuying@cau.edu.cn.
Chuduan WangState Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P. R. China. cdwang@cau.edu.cn.ORCID http://orcid.org/0009-0009-7826-5467

Funding

National Key R&D Program of China No. 2023YFD1300400STI 2030-Major Projects No. 2023ZD04046
6 · The paper itself

Abstract

backgroundViral diseases have profoundly influenced the sustainable development of the swine farming industry. With the development of genomics technology, the combination of transcriptome, genetic variation, immune response, and QTL mapping data to illustrate the interactions between pathogen and host immune system, will be an effective tool for identification of disease resistance genes in pigs. The immune system of an organism is the source of disease resistance in livestock, consisting of various immune tissues, as well as the immune cells and cytokines they produced. However, comprehensive systematic studies on transcriptome of porcine immune tissues are still rare. Poly(I:C), as a viral mimic, is commonly used to study immune responses of the body during viral infections, and serves as a valuable tool for investigating immune mechanisms in swine.

resultsWGCNA analysis identified core immune genes across six immune tissues (bone marrow, jejunum, lymph node, PBMC, spleen, thymus) in Landrace pigs, which are also crucial for the development of PBMCs. The examination of the changes in the proportion of immune cells during three developmental stages (1-month-old, 4-month-old, 7-month-old) shows a shift from innate immunity to humoral immunity. By integrating different epigenetic genomics datasets, we identified several core immune genes and their causal variants, including IFI44, IFIT5, EIF2AK2 and others, which are closely related to immune development and response. Functional validation studies reveal that the IFI44 gene acts as a negative regulator of the antiviral response; its inhibition effect significantly reduced Poly(I:C)-induced cell necrosis, while enhancing apoptosis to combat viral infections.

conclusionOur study elucidated the fundamental transcriptional program in porcine immune tissues and the immunodynamics underlying development of PBMCs, identifying many core immune genes, including IFI44, which plays a critical negative regulator role in the antiviral response, providing valuable insights for breeding programs aimed at enhancing pig disease resistance.

Indexed as

Antiviral responseCore immune genesImmune tissuesPBMCPoly(I:C)

Identifiers

PMID40390102
PMCPMC12087129

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.