ArticleNature communications2025
LLPS REDIFINE allows the biophysical characterization of multicomponent condensates without tags or labels.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A New Customizable Surfactant LLPS Strategy for Sustainable and Highly Efficient Radioactive Metal Ion Separation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Dysregulation of liquid-liquid phase separation: from molecular mechanisms to targeted therapies in cardiovascular diseases.Journal of nanobiotechnology · 2026Review
- Quantifying Transmembrane Water Exchange by Diffusion NMR Methods: From Yeast Cells to Optic Nerve Ex Vivo.NMR in biomedicine · 2026Article
- Bioresponsive immunomodulator nanocomplex for selective immunoengineering in metastatic lymph nodes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Beyond structures: solution NMR as the quantitative engine of integrated structural biology.Frontiers in molecular biosciences · 2026Review
- Multiplexed Analysis of Multicomponent Biomolecular Condensates without Any Tag.Journal of the American Chemical Society · 2025Article
- RNA-Mediated Inhibition Mechanism of Liquid-Liquid Phase Separation and Subsequent Aggregation Revealed by Raman Microscopy.JACS Au · 2025Article
- Article
- Water-detected NMR allows dynamic observations of repeat-expansion RNA condensates.Nature chemistry · 2025Article
- Selective Ion Binding and Uptake Shape the Microenvironment of Biomolecular Condensates.Journal of the American Chemical Society · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
Liquid-liquid phase separation (LLPS) phenomenon plays a vital role in multiple cell biology processes, providing a mechanism to concentrate biomolecules and promote cellular reactions locally. Despite its significance in biology, there is a lack of conventional techniques suitable for studying biphasic samples in their biologically relevant form. Here, we present a label-free and non-invasive approach to characterize biomolecular condensates termed LLPS REstricted DIFusion of INvisible speciEs (REDIFINE). Relying on diffusion NMR measurements, REDIFINE exploits the exchange dynamics between molecules in the condensed and dispersed phases to determine not only diffusion constants and the fractions in both phases but also the average radius of the condensed droplets and the exchange rate between the phases. Observing proteins, RNAs, water, as well as small molecules, and even assessing the concentrations of biomolecules in both phases, REDIFINE analysis allows a rapid biophysical characterization of multicomponent condensates which is important to understand their functional roles. In comparing multiple systems, REDIFINE reveals that folded RNA-binding proteins form smaller and more dynamic droplets compared to the disordered ones.
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