Evidence map›Paper›PMID 40389054›Full record

ArticleDevelopmental biology2025

Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide binding.

Martha L Echevarría-Andino, Jane Y Song, Paul R van Ginkel, Shuyang Chen, Corey G K Flynn, Sündüz Keleş, Benjamin L Allen, Deneen M Wellik

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Martha L Echevarría-AndinoDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: mlechevarria@wisc.edu.
Jane Y SongDepartment of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: jysong2@wisc.edu.
Paul R van GinkelDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: prvangin@wisc.edu.
Shuyang ChenDepartment of Statistics, University of Wisconsin-Madison, Madison, WI, 53706, USA. Electronic address: schen662@wisc.edu.
Corey G K FlynnDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: cflynn9@wisc.edu.
Sündüz KeleşDepartment of Statistics, University of Wisconsin-Madison, Madison, WI, 53706, USA; Department of Biostatistics and Medical Informatics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: keles@biostat.wisc.edu.
Benjamin L AllenDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109, USA. Electronic address: benallen@umich.edu.
Deneen M WellikDepartment of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Electronic address: wellik@wisc.edu.

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Eric R. Fearon · 1988 to 2026
$178.2M
University of Michigan Postbaccalaureate Research Education Program (UM PREP)R25GM086262 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALLEN, BENJAMIN · 2009 to 2024
$6.1M
Statistical Methods for the Analysis of ChlP-chip DataR01HG003747 · NHGRI · UNIVERSITY OF WISCONSIN-MADISON · PI KELES, SUNDUZ · 2007 to 2024
$4.8M
Hox-Regulated MSCs in Skeletal Development, Growth and Fracture HealingR37AR061402 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI Deneen M Wellik · 2022 to 2026
$2.5M
NCI NIH HHS P30 CA046592NHGRI NIH HHS R01 HG003747NIAMS NIH HHS R37 AR061402NIGMS NIH HHS R25 GM086262
6 · The paper itself

Abstract

Hox genes encode for evolutionary conserved transcription factors that direct the proper development of the body plan. Despite decades of research, little is known regarding their downstream target genes, especially in vertebrates. The strong evolutionary conservation of their DNA-binding homeodomain, their generic AT-rich binding sites, and the lack of specific antibodies has precluded rigorous examination. To circumvent these limitations, we have generated two mouse models in which a 3XFLAG epitope tag has been inserted into the 5' end of the coding sequence of both Hoxa11 and Hoxd11 loci via Cas9/CRISPR. The alleles have been validated by sequencing, PCR genotyping, western blotting, and protein expression analyses, demonstrating proper targeting and expression. Breeding these alleles in combination produces viable and fertile Hoxa11

Indexed as

Homeodomain ProteinsTranscription FactorsAllelesAnimalsFemaleGene Expression Regulation, DevelopmentalGenomeKidneyMiceHomeodomain ProteinsHoxa11 protein, mouseSix2 protein, mouseTranscription Factors3XFLAGEpitope-tagged allelesHox genesHoxPG11

Identifiers

PMID40389054
PMCPMC12189421

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.