ArticlePloS one2025
Validation of dietary whey protein substrate challenge absorption test as an indicator of proteolysis.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Blood-based biomarkers of protein digestibility, utilization, and nitrogen excretion in animals: Concepts, evidence, and applications.Veterinary and animal science · 2026Review
- Dependency of Glucose Homeostasis on Pancreatic Enzymes with Special Reference to Amylase; Study on Healthy and Exocrine Pancreatic Insufficient Pigs.Biomolecules · 2026Article
- Prospects for fistula technologies in the study of digestion and metabolism in birds.Frontiers in physiology · 2026Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
introductionCurrent methods to measure the effectiveness of pancreatic protease activity are inadequate. We explored the measurement of peptide-derived amino acids following ingestion of dietary whey substrate as a sensitive test of exogenous protease activity in exocrine pancreatic insufficient (EPI) pigs.
methodsWe studied the activity of aspergillus protease given in combination with a novel lipase and fungal amylase, as well as commercially available pancrelipase in EPI pigs. After a high-fat diet plus a standardized dietary whey substrate, blood was withdrawn at intervals and was analyzed for amine groups using a modified ninhydrin reaction. Plasma peptide-derived amino acids were calculated.
resultsThe AUC6 peptide-derived amino acid concentration was significantly increased in response to aspergillus protease as follows: 50 mg dose (137% increase; p = 0.05), 75 mg dose (154% increase; p = 0.008) compared to no enzyme. The AUC6 for peptide-derived amino acids after aspergillus protease increased by 133% for the 50 mg dose (p = 0.0044), by 171% for the 75 mg dose (p = 0.0002), and by 113% with 600 mg pancrelipase (p < 0.0001) when compared to no enzyme. Administration of 75 mg of aspergillus protease led to significantly higher peptide-derived amino acid AUC6 and Cmax when compared to 600 mg pancrelipase (p = 0.0419 and 0.0103, respectively).
conclusionIn EPI pigs, measurement of peptide-derived amino acids following a meal with whey substrate differentiates the activity of aspergillus protease compared to no enzyme and the 75 mg dose was superior to pancrelipase. The evidence presented here in EPI pigs demonstrates that the whey substrate absorption challenge test reflects the proteolytic activity of different doses of exogenous pancreatic proteases.
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