Evidence map›Paper›PMID 40388240›Full record

ArticleVeterinary medicine and science2025

Expression and Immunogenicity Analysis of Recombinant Leptospira Interrogans Surface Protein LigA in Mouse Model.

Aida Chalesh, Pejvak Khaki, Soheila Moradi Bidhendi, Majid Tebianian, Morteza Taghizadeh Tarnabi

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Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Aida ChaleshDepartment of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.ORCID 0009-0001-5850-0812
Pejvak KhakiDepartment of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.ORCID 0000-0001-8839-1023
Soheila Moradi BidhendiDepartment of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.ORCID 0000-0003-4041-6257
Majid TebianianDepartment of Immunology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Morteza Taghizadeh TarnabiDepartment of Medical Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Funding

Razi Vaccine and Serum Research Institute 12-18-18-106-96045-961025
6 · The paper itself

Abstract

backgroundPathogenic strains of spirochetes of Leptospira spp. cause a globally distributed zoonotic disease called leptospirosis. The disease has several clinical manifestations, ranging from asymptomatic and subclinical infection to fatal and severe forms. HYPOTHESIS/

objectivesThe aim of this study was to produce a recombinant Leptospiral immunoglobulin-like surface protein-A (r-LigA) antigen of Leptospira interrogans in a prokaryotic expression system and to assess its efficacy in a mouse model. MATERIALS AND

methodsThe optimal epitopes of the LigA protein were identified via bioinformatics studies. The pET32a

resultsBioinformatics analysis identified the key antigenic region of LigA spanning amino acids 852 to 1210. Colony PCR and digestion confirmed the successful transformation. Induction using 0.5 mM IPTG at 30°C for 5 h was found to be optimal. Overexpression of r-LigA under optimised conditions accumulated proteins as inclusion bodies. Purification of r-LigA under native conditions using optimised Ni-NTA yielded 1050 µg/mL protein and high immunogenicity by effectively stimulating the immune system in female Balb/C mice.

conclusionsThese findings support r-LigA as a strong candidate for future leptospirosis diagnostic tools and subunit vaccine development.

Indexed as

Antigens, BacterialBacterial VaccinesLeptospira interrogansLeptospirosisAnimalsDisease Models, AnimalFemaleMiceMice, Inbred BALB CRecombinant ProteinsAntigens, BacterialBacterial VaccinesLigA-m protein, Leptospira interrogansRecombinant Proteinsimmune systemLeptospira interrogansLeptospirosisNi‐NTA affinity purificationrecombinant Leptospiral immunoglobulin‐like surface protein A

Identifiers

PMID40388240
PMCPMC12087437

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