Evidence map›Paper›PMID 40388033›Full record

ArticleMolecular biology reports2025

Comparative analysis of rooting abilities between cuttings of trifoliate orange and Micro-Tom tomato: hormonal and molecular insights into adventitious root formation.

Abebe Assefa Gobena, Abdelmonem Elshahat, Hai-Qiang Liu, Dong-Hai Liu, Han Han, Yin Luo, Shariq Mahmood Alam, Muhammad Ateeq, Muhammad Abbas Khan, Yong-Zhong Liu

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Abebe Assefa GobenaNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Abdelmonem ElshahatNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Hai-Qiang LiuNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Dong-Hai LiuNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Han HanNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Yin LuoNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Shariq Mahmood AlamNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Muhammad AteeqNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Muhammad Abbas KhanNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Yong-Zhong LiuNational Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. liuyongzhong@mail.hzau.edu.cn.

Funding

Earmarked Fund for CARS-26, Hubei International Scientific and Technological Cooperation Program 2022EHB032Huazhong Agricultural University Seed Fund 901/11050020115
6 · The paper itself

Abstract

backgroundThe trifoliate orange is used as rootstock in the Citrus genus; nevertheless, propagation from cuttings is challenging due to low rooting ability. The underlying mechanism for low rooting ability is still unclear. This study aims to investigate hormonal biosynthesis and signaling cascades during Adventitious Root (AR) formation. METHODS AND

resultsWe systematically compared trifoliate orange and Micro-Tom tomato cuttings grown in water. The results indicated Micro-Tom tomato cuttings produced ARs within 3 days, whereas trifoliate orange developed ARs at 21 days after cuttings (DAC). The key endogenous hormones contents: indole-3-acetic acid (IAA), Jasmonic acid (JA), and salicylic acid (SA), were significantly lower in trifoliate orange than Micro-Tom tomato cuttings at earlier stages 1, 2, and 3 DAC. Moreover, IAA-related and ethylene biosynthesis genes demonstrated differential expression patterns during different developmental stages between trifoliate orange and Micro-Tom tomato. Specially, the expression levels of auxin biosynthesis genes (YUCCA6/4, GH3.3/3.6) and response factor (ARF8) were significantly elevated in Micro-Tom tomato cuttings, while lower in trifoliate orange cuttings at the earliest stages. Additionally, transcripts related to auxin signaling and transport (PIN2, LAX2, AUX1, TR2a, IAA11/12) and ethylene-related transcripts (ACS3/7, ASA1, ERF003/109) were lower in trifoliate orange than Micro-Tom tomato cuttings during the earliest stages.

conclusionsOur results confirmed AR formation in trifoliate orange was delayed compared to Micro-tom tomato. The low level IAA hormones, significantly lower expression IAA and ethylene-related genes at the earliest stages could be attributed to the delay of AR formation in trifoliate orange cuttings.

Indexed as

CitrusCitrus sinensisPlant Growth RegulatorsPlant RootsSolanum lycopersicumCyclopentanesEthylenesGene Expression Regulation, PlantIndoleacetic AcidsOxylipinsPlant ProteinsSalicylic AcidCyclopentanesethyleneEthylenesindoleacetic acidIndoleacetic Acidsjasmonic acidOxylipinsPlant Growth RegulatorsPlant ProteinsSalicylic AcidAdventitious rootCuttingsGenesHormonesMicro-Tom tomatoTrifoliate

Identifiers

PMID40388033

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.