Evidence map›Paper›PMID 40387079›Full record

ArticleeLife2025

Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum.

Lin Wei, Hai-Yan Cao, Ruyi Zou, Min Du, Qingdong Zhang, Danrong Lu, Xiangyu Xu, Yingying Xu, Wenshuang Wang, Xiu-Lan Chen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lin Wei *National Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.ORCID https://orcid.org/0000-0002-9348-9508
Hai-Yan Cao *MOE Key Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, Qingdao, China.ORCID https://orcid.org/0000-0003-1365-2094
Ruyi ZouNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Min DuNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Qingdong ZhangNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Danrong LuNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Xiangyu XuNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Yingying XuNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Wenshuang WangNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Xiu-Lan ChenMarine Biotechnology Research Center, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Yu-Zhong ZhangMOE Key Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, Qingdao, China.ORCID https://orcid.org/0000-0002-2017-1005
Fuchuan LiNational Glycoengineering Research Center and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.ORCID https://orcid.org/0000-0003-1920-9691

Funding

Major Scientific and Technology Innovation Project (MSTIP) of Shandong Province 2019JZZY010817National Key Research and Development Program of China 2024YFC2816005National Natural Science Foundation of China 31570071National Natural Science Foundation of China 31800665National Natural Science Foundation of China 31971201National Natural Science Foundation of China 32330001Natural Science Foundation of Shandong Province ZR2023MC017Natural Science Foundation of Shandong Province ZR2024MD015Shandong Provincial Youth Innovation Science and Technology Support Program for Colleges and Universities 2022KJ003State Key Laboratory of Microbial Technology Open Projects Fund M2024-15The SKLMT Frontiers and Challenges Project SKLMTFCP-2023-06
6 · The paper itself

Abstract

Recently, a new class of glycosaminoglycan (GAG) lyases (GAGases) belonging to PL35 family has been discovered with an ultrabroad substrate spectrum that can degrade three types of uronic acid-containing GAGs (hyaluronic acid, chondroitin sulfate and heparan sulfate) or even alginate. In this study, the structures of GAGase II from

Indexed as

BacteroidesGlycosaminoglycansPolysaccharide-LyasesBacterial ProteinsCrystallography, X-RayModels, MolecularMolecular Docking SimulationProtein ConformationSubstrate SpecificityBacterial ProteinsGlycosaminoglycansPolysaccharide-Lyasesbiochemistrycatalytic mechanismchemical biologycrystal structureglycosaminoglycan lyasesnonepolysaccharide lyase 35 family

Identifiers

PMID40387079
PMCPMC12088678

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.