ArticleArtificial organs2025
Development of a Protocol for Long-Term Ex Vivo Normothermic Machine Perfusion of Rodent Livers.
Article in Artificial organs, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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10 authors.
Funding
Abstract
introductionLong-term normothermic machine perfusion (LT-NMP) enables the assessment and optimization of livers for days and, potentially, weeks. However, models of LT-NMP have only been described for human and pig livers, which are resource intensive and impractical for laboratory research. Cost-effective small animal models of LT-NMP are needed for future research. This study aimed to develop a system for LT-NMP of rat livers for up to 72 h.
methodsThis study was performed in two stages: the development phase (n = 20) and validation phase (n = 5). The perfusion system included an organ reservoir, pump, heat exchanger, long-term oxygenator, and dialysis. Hormonal and nutritional support were continuously infused. During the validation phase, five consecutive grafts were perfused using our protocol. At 72 h postreperfusion, grafts were assessed for viability, which was based on hemodynamic stability, mitochondrial function, bile production, and metabolic activity.
resultsRodent livers were supported up to 107 h using our LT-NMP protocol. All grafts in the validation phase remained viable at 72 h (n = 5/5). The median oxygen consumption and bile production at 72 h were 0.079 mLO
conclusionsThis is the first study to report LT-NMP of rodent livers up to 5 days. Using our protocol, rat livers could reliably be supported until 72 h. This model provides a greater opportunity to investigate novel therapeutics to assess, optimize, and regenerate liver grafts for transplantation.
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