Investigating the fluorescence in C-dots immobilised on alginate hydrogels-a study on diffusion kinetics and adsorption mechanisms.

Immobilisation of fluorescent carbon dots (C-dots) in a hydrogel matrix, such as alginates, prevents fluorescence quenching in bioimaging and biosensing applications. However, critical parameters influencing the fluorescence, including the diffusion kinetics of C-dots and their distribution within the hydrogel matrix, remain unexplored. Herein, we investigated two distinct methods for immobilising C-dots within alginate hydrogel beads: (i) adsorption and (ii) premixing the C-dots prior to hydrogel cross-linking. Our batch adsorption experiments and kinetic model fittings revealed rapid, concentration-dependent diffusion from the external solution to the beads, along with their binding to surface active sites. The rate-determining step was the diffusion into interconnecting layers within the matrix, which impacts both diffusion rates and the overall distribution of C-dots within the beads. The fluorescence signal in the hydrogel matrix from the adsorption method exhibited limited penetration depth compared to the premixed method, which showed a more uniform distribution. We demonstrated that C-dots are well-immobilised and interact effectively with the hydrogel matrix, exhibiting stable fluorescence intensities and improved structural integrity. Our findings provide valuable insights into the interaction and diffusion of C-dots in hydrogel systems and will help advance research on the fluorescence properties of C-dots for bioimaging and bio-sensing applications.