ArticleFrontiers in microbiology2025
Unveiling the impact of 16S rRNA gene intergenomic variation on primer design and gut microbiome profiling.
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Salivary microbial meta-analysis reveals gender differences in oral microbiota, core microbiota, and molecular markers.Frontiers in cellular and infection microbiology · 2026Pooled it
- Moving from a taxonomic to a functional perspective in global microbiome analysis requires optimizing multiplexing ratios.mSystems · 2026Article
- Ameliorative Effects of an Antioxidant and Anti-Inflammatory Limosilactobacillus fermentum TG017 on DSS-Induced Ulcerative Colitis in Mice.Probiotics and antimicrobial proteins · 2026Article
- Detection and Molecular Identification of Phytoplasmas Associated with Potato in Iran.Microorganisms · 2026Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 16S rRNA gene is crucial for bacterial identification, but primer biases and intergenomic variation can compromise its effectiveness, especially in complex ecosystems like the human gut microbiome. This study systematically evaluates 57 commonly used 16S rRNA primer sets through
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.