ArticleMolecular pharmaceutics2025
Comparative Analysis of mRNA Degradation Kinetics Using Chromatographic and Electrophoretic Methods.
Article in Molecular pharmaceutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Review
- Preparation and Immunogenicity Prediction ofVaccines · 2026Article
- Scatter-Free UV-Visible Spectroscopy for Accurate and Precise RNA Quantification in Complex RNA Nanoparticle Formulations.Analytical chemistry · 2025Article
- Key Clinical Frontiers of mRNA Loaded Lipid Nanoparticles in Cancer Vaccines.International journal of nanomedicine · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of temperature and lipid nanoparticle (LNP) encapsulation on mRNA chemical stability were assessed for two model mRNA (EGFP mRNA, 996 nt; Fluc mRNA, 1929 nt) using capillary electrophoresis (CE) and ion pair reversed phase high performance liquid chromatography (IP-RP-HPLC) to measure mRNA integrity. The apparent degradation rates for unencapsulated ("naked") mRNA were method-dependent for Fluc mRNA, with rates up to 50% greater when measured using CE compared to IP-RP-HPLC. This discrepancy is attributed to differences in mass resolution, separation mechanisms and/or linearity of the two methods and was not observed for EGFP mRNA. The apparent reaction order for naked mRNA degradation varied with storage temperature, being zero-order at 35 °C and first-order at 50 °C. Encapsulation of mRNA using LNPs slowed mRNA degradation by up to 9-fold relative to naked mRNA controls stored under the same conditions. Encapsulation also induced changes in thermal unfolding patterns. Overall, the results show the benefits and limitations of each analytical method for determining mRNA degradation kinetics and the effects of temperature and LNP encapsulation on mRNA stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.