Evidence map›Paper›PMID 40383590›Full record

ArticleCarbohydrate polymers2025

Evaluating size exclusion chromatography for nucleic acid removal in Klebsiella pneumoniae cell surface polysaccharide purification.

Kanti N Mihooliya, Ambrish Kumar, Christian Heiss, Alka Kumari, Camilla Boniche-Alfaro, Parastoo Azadi, Bettina C Fries

Abstract read
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In one paragraph

Article in Carbohydrate polymers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kanti N MihooliyaDivision of Infectious Diseases, Department of Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, USA; Veteran's Administration Medical Center, Northport, New York, USA.
Ambrish KumarComplex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA 30602, USA.
Christian HeissComplex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA 30602, USA.
Alka KumariDivision of Infectious Diseases, Department of Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, USA.
Camilla Boniche-AlfaroDivision of Infectious Diseases, Department of Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, USA; Veteran's Administration Medical Center, Northport, New York, USA.
Parastoo AzadiComplex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA 30602, USA.
Bettina C FriesDivision of Infectious Diseases, Department of Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, USA; Veteran's Administration Medical Center, Northport, New York, USA; Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, USA. Electronic address: bettina.fries@stonybrookmedicine.edu.

Funding

National Glycoscience Resource- CCRC Service and Training - NGlycoR@CCRCR24GM137782 · NIGMS · UNIVERSITY OF GEORGIA · PI Parastoo Azadi · 2020 to 2026
$5.2M
BLRD VA I01 BX003741NIGMS NIH HHS R24 GM137782
6 · The paper itself

Abstract

Cell surface-associated polysaccharides in Klebsiella are major virulence determinants and crucial targets for developing vaccines. Traditionally, the purification of these cell surface polysaccharides from Klebsiella pneumoniae involves a multi-step process comprising phenol extraction, nuclease digestion, ultracentrifugation, and repeated ethanol extractions. In this study, we evaluated size exclusion chromatography for effectively eliminating nucleic acid contamination while purifying high molecular weight cell surface-associated polysaccharides. Post-initial extraction, the nucleic acid content remains significantly elevated, and kinetic analysis reveals that DNase I and RNase A digestion is neither economically viable nor effective for removing these contaminants. Employing an appropriate size exclusion resin removes over 99 % of nucleic acid contamination, as confirmed by nucleic acid content analysis and agarose gel electrophoresis. Purity and structural analysis using

Indexed as

Chromatography, GelKlebsiella pneumoniaeNucleic AcidsPolysaccharides, BacterialNucleic AcidsPolysaccharides, BacterialCell surface polysaccharidesKlebsiella pneumoniaeNuclear magnetic resonance (NMR)Nucleic acids (NA)Size exclusion chromatography (SEC)Vaccine targets

Identifiers

PMID40383590
PMCPMC12236454

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.