Evidence map›Paper›PMID 40382553›Full record

ArticleBMC plant biology2025

Beyond nitrate transport: AtNRT2.4 responds to local and systemic nitrogen signaling in Arabidopsis.

Huaifang Zhang, Jinxia Huang, Yanmei Li, Jinling Zhao, Weitao Mai, Luqman Khan, Meng Zhang, Wenquan Wang, Changying Zeng, Xin Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huaifang ZhangSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Jinxia HuangInstitute of Tropical Bioscience and Biotechnology/Sanya Research Institute, Chinese Academy of Tropical Agricultural Science, Haikou, China.
Yanmei LiSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Jinling ZhaoSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Weitao MaiSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Luqman KhanSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Meng ZhangLiaoning Academy of Agricultural Sciences, Shenyang, China.
Wenquan WangSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China.
Changying ZengSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China. zengchangying@hainanu.edu.cn.
Xin ChenSanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry, National Key Laboratory for Tropical Crop Breeding, Hainan University, Sanya, China. chenxin@itbb.org.cn.

Funding

Graduate Innovation Project of Hainan Province Qhyb2022-57Hainan Province Science and Technology Special Fund ZDYF2023XDNY179Hainan University Research Star-up Funding RZ2100003186National Natural Science Foundation of China 32060714Project of National Key Laboratory for Tropical Crop Breeding NKLTCBCXTD07
6 · The paper itself

Abstract

backgroundPlants have evolved the ability to detect nitrogen fluctuations to optimize their nitrogen acquisition. However, the mechanisms of nitrogen perception and signaling still need to be well characterized.

resultsThrough split-root experiments, this study demonstrated that nitrate transporter 2.4 (AtNRT2.4) can respond to both local and systemic nitrate signals, modulating the transcription of genes such as AtANR1 and AtCIPK23, thereby altering root architecture. Beyond merely detecting the fluctuations of environmental nitrate concentrations, AtNRT2.4 was actively engaged in the dual-affinity transition of AtNRT1.1 and suppressed the expression of AtNLP7, which is crucial for responding to intracellular nitrate signals. Notably, AtNRT2.4 did not participate in the CEP-mediated systemic nitrogen stress signaling pathway and also did not require AtNRT3.1 as a chaperone protein. The knockout of AtNRT2.4 did not affect the growth of Arabidopsis thaliana under low nitrate conditions. However, its overexpression significantly enhanced biomass accumulation and seed yield under normal nitrate concentrations. Furthermore, under nitrate deficiency stress, AtNRT2.4 induced the expression of key genes involved in anthocyanin synthesis and accumulation, thereby promoting anthocyanin accumulation in leaves.

conclusionsIn summary, AtNRT2.4 plays a crucial role in local and systemic nitrate signals sensing, adjustment of root architecture, and anthocyanin accumulation, providing new insights into how plants respond to nitrogen deprivation.

Indexed as

Anion Transport ProteinsArabidopsisArabidopsis ProteinsNitratesNitrogenSignal TransductionBiological TransportGene Expression Regulation, PlantNitrate TransportersPlant RootsAnion Transport ProteinsArabidopsis ProteinsNitratesNitrate TransportersNitrogenAnthocyanin accumulationLocal N-deficiency signalsNitrate deficiencyRoot architectureSystemic nitrate signals

Identifiers

PMID40382553
PMCPMC12084985

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.