ArticleSTAR protocols2025
Protocol to chemically deplete phagocytic hemocytes in Anopheles gambiae using clodronate liposomes.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Characterization of Anopheles gambiae immune cells through genetic and functional immunophenotyping.Nature communications · 2025Article
- A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes.Nature communications · 2025Article
- Phagocytic hemocytes, independent of pericardial cells, modulate cellular immune responses on the dorsal vessel of mosquitoes, including the infection-induced reduction of the heart rate.Cell and tissue research · 2025Article
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Authors and funding
2 authors.
Funding
Abstract
Understanding the roles of phagocytic hemocytes in mosquito innate immunity has been significantly limited due to the lack of genetic tools. Here, we present a protocol for depleting phagocytic hemocytes in Anopheles gambiae mosquitoes using clodronate liposomes. We describe steps for mosquito injection, as well as validation by microscopy, quantitative real-time PCR (real-time qPCR), and flow cytometry analysis. This protocol allows for the delineation of phagocytic hemocyte function in mosquito immunity, which can be more broadly applied to other arthropod systems. For complete details on the use and execution of this protocol, please refer to Kwon et al.
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