Evidence map›Paper›PMID 40381002›Full record

ReviewHistochemistry and cell biology2025

Immunocytochemical detection of proteins within cellular structures inaccessible to specific antibodies.

Maria A Tikhomirova, Anastasia A Zharikova, Yana R Musinova, Eugene V Sheval

Abstract readReview
PubMed Publisher
In one paragraph

Review in Histochemistry and cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. June in focus in HCB.Histochemistry and cell biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maria A TikhomirovaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Anastasia A ZharikovaSchool of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119991, Russia.
Yana R MusinovaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.ORCID http://orcid.org/0000-0002-0790-5510
Eugene V ShevalBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia. sheval_e@belozersky.msu.ru.ORCID http://orcid.org/0000-0003-1687-1321

Funding

Russian Science Foundation 24-24-00168
6 · The paper itself

Abstract

Immunocytochemistry and immunohistochemistry are among the most widely used and valuable methods for localizing and quantifying proteins in cells and tissues. However, these methods have several limitations, and they are still being improved. A notable problem in this area is the restricted accessibility of some dense intracellular structures, such as nucleoli, to specific antibodies. This reduced accessibility led to peripheral staining of the nucleoli even in cases of relatively homogeneous distribution of the antigen within the nucleoli. This review aims to elucidate the factors impeding antibody accessibility to the internal regions of the nucleolus in somatic cells and nucleolus-like bodies in oocytes and to explore the methods used to circumvent this limitation.

Indexed as

AntibodiesImmunohistochemistryProteinsAnimalsHumansAntibodiesProteinsAntibodyAntigen retrievalImmunocytochemistryNucleolar rimNucleolusNucleolus-like body

Identifiers

PMID40381002

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.