Evidence map›Paper›PMID 40380997›Full record

ArticleSurgery today2025

Induced pluripotent stem cell-derived stellate cells promote proliferation of induced pluripotent stem cell-derived hepatocytes through the mitogen-activated protein kinase pathway via hepatocyte growth factor.

Takahiro Tomiyama, Kazuki Takeishi, Shinji Itoh, Katsuya Toshida, Norifumi Iseda, Yuki Nakayama, Takuma Ishikawa, Takashi Motomura, Takeshi Kurihara, Takeo Toshima and 3 more

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Article in Surgery today, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Takahiro TomiyamaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Kazuki TakeishiDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan. takeishi.kazuki.963@m.kyushu-u.ac.jp.
Shinji ItohDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Katsuya ToshidaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Norifumi IsedaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Yuki NakayamaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Takuma IshikawaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Takashi MotomuraDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Takeshi KuriharaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Takeo ToshimaDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.
Rodrigo M FlorentinoDepartment of Pathology, School of Medicine, University of Pittsburgh, 200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA, 15261, USA.
Alejandro Soto-GutierrezDepartment of Pathology, School of Medicine, University of Pittsburgh, 200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA, 15261, USA. als208@pitt.edu.
Tomoharu YoshizumiDepartment of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka, 812-8582, Japan.

Funding

Japan Society for the Promotion of Science 21H03004
6 · The paper itself

Abstract

purposeHuman-induced pluripotent stem cells (iPSCs) have the potential to differentiate into cells of various organs. Hepatocytes derived from iPSCs (i-Heps) have attracted much attention as an alternative treatment for liver transplantation in patients with liver failure. However, it is challenging to create sufficient i-Heps for clinical treatment, highlighting the need to develop an easier and more efficient culture method. In this study, we examined the effect of quiescent iPSC-derived stellate cells (i-Stes) on i-Hep proliferation.

methodsi-Stes and i-Heps were differentiated from iPSCs.

resultsi-Stes expressed higher levels of hepatocyte growth factor (HGF) than the human hepatic stellate cell line LX-2. In addition, quiescent i-Sted promoted i-Hep proliferation via activation of the mitogen-activated protein kinase (MAPK) pathway in i-Heps, which was impaired by the activation of i-Sted with transforming growth factor beta.

conclusionThis study provides evidence that i-Sted can effectively induce i-Hep proliferation through HGF activation of the MAPK signaling pathway. Quiescent-but not activated-i-Stes may contribute to the creation of large numbers of i-Heps.

Indexed as

Cell DifferentiationCell ProliferationHepatic Stellate CellsHepatocyte Growth FactorHepatocytesInduced Pluripotent Stem CellsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesCell LineCells, CulturedHumansHepatocyte Growth FactorMitogen-Activated Protein KinasesHepatocyte proliferationHuman-induced pluripotent stem cell (iPSC)IPSC-derived hepatocyteIPS-derived stellate cell

Identifiers

PMID40380997

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.