ArticleMethods in molecular biology (Clifton, N.J.)2025
A Visual Reporter System for Analyzing Small RNA-Triggered Local and Systemic Silencing of an Endogenous Plant Gene.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.Nucleic acids research · 2026Article
- Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing.The Plant journal : for cell and molecular biology · 2026Article
- Gene silencing in plants by artificial small RNAs derived from minimal precursors and expressed via tobacco rattle virus.Plant molecular biology · 2025Article
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Authors and funding
2 authors.
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Abstract
Plant small RNAs (sRNAs) silence highly sequence complementary target mRNAs locally in a cell-autonomous manner but can also move long distances to induce systemic gene silencing in distal tissues. Unfortunately, the lack of appropriate experimental systems has hampered the identification of the factors determining the induction and spread of local and systemic gene silencing triggered by plant sRNAs. Here we describe a visual reporter system in which both local and systemic silencing of Nicotiana benthamiana SULFUR (NbSu) gene is triggered by the agroinfiltration of an artificial sRNA construct. Agroinfiltrated areas show strong and uniform bleaching while apical non-agroinfiltrated leaves display a characteristic near-vein chlorosis. This visual reporter system should facilitate the identification of the genetic, molecular, and/or environmental factors regulating both local and systemic silencing activities of plant sRNAs.
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