ArticleMethods in molecular biology (Clifton, N.J.)2025
MicroRNA Profiling in Non-model Plants Using Microarray Hybridization.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The aim of this chapter is to describe how microarrays can be used to identify miRNAs that participate in fruit development in a non-model plant: prickly pear (Opuntia spp). Here, we describe a technology including molecular biology and bioinformatics methods combined to identify, select, and isolate candidate miRNAs putatively involved in prickly pear development. We describe how miRNA expression analysis through microarray hybridization was performed and the expression of selected miRNAs validated using RT-PCR.
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