ArticleMethods in molecular biology (Clifton, N.J.)2025
Northern Blot Analysis of Plant MicroRNAs and Other Small RNAs.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors.Bio-protocol · 2025Article
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3 authors.
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Abstract
The study of regulatory small RNAs, such as siRNAs and microRNAs in plants and other organisms, has necessitated techniques tailored to their unique features. Their analysis requires the use of sensitive and quantitative methods for their detection. The use of northern blot hybridization offers an attractive approach to address qualitative as well as quantitative features, and we describe here the use of radioactive and nonradioactive methods for signal detection. We highlight the advantages and shortcomings of these approaches and offer a detailed description of the techniques that best work for us, considering their use for the study of various small RNAs in multiple samples. We list relevant details and provide cautionary comments in those steps where we have detected potential difficulties.
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Registered trials
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