ArticleNature communications2025
CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Integrating multitemperature sample preparation with particle rebalancing addresses preferred orientation.PNAS nexus · 2026Article
- UsingbioRxiv : the preprint server for biology · 2026Article
- Overcoming the challenge of preferred particle orientation in cryo-EM.Nature reviews. Molecular cell biology · 2026Article
- A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM.bioRxiv : the preprint server for biology · 2026Article
- A comprehensive foundation model for cryo-EM image processing.Nature methods · 2026Article
- Nanoscale Imaging of Biological Tissues: Techniques, Challenges and Emerging Frontiers.Nanomaterials (Basel, Switzerland) · 2025Review
- CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology.bioRxiv : the preprint server for biology · 2025Article
- Laser flash melting cryo-EM samples to overcome preferred orientation.Nature methods · 2025Article
- CryoDataBot: a pipeline to curate cryoEM datasets for AI-driven structural biology.GigaScience · 2025Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The preferred orientation phenomenon is a common issue in cryo-EM, posing a persistent challenge to conventional reconstruction methods. In this study, we introduce cryoPROS, a computational framework designed to correct misalignment caused by preferred orientation through co-refining the raw and auxiliary particles. These auxiliary particles, generated using a self-supervised deep generative model, enhance the alignment accuracy of particles in datasets affected by preferred orientation. CryoPROS achieved near-atomic resolution with the untilted HA-trimer dataset and successfully resolved high-resolution structures from three experimental datasets, including P001-Y, Na
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Registered trials
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