Evidence map›Paper›PMID 40377475›Full record

ArticleThe Journal of cell biology2025

Cargo adaptors use a handhold mechanism to engage with myosin V for organelle transport.

Hye Jee Hahn, Natalya Pashkova, Michael A Cianfrocco, Lois S Weisman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Hye Jee HahnDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0001-9389-6034
Natalya PashkovaDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.ORCID 0000-0002-2191-4683
Michael A CianfroccoLife Sciences Institute, University of Michigan , Ann Arbor, MI, USA.ORCID 0000-0002-2067-4999
Lois S WeismanDepartment of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0001-7740-9785

Funding

Regulation of myosin V interaction with cargo-Equipment SupplementR01GM062261 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lois S Weisman · 2001 to 2026
$5.8M
Compute Cluster for in vitro and in situ Analysis of Molecular MachinesS10OD030275 · OD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI OHI, MELANIE DIANE · 2021 to 2021
$600k
Silicon Mechanics Compute ClusterS10OD020011 · OD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SKINIOTIS, GEORGIOS · 2015 to 2015
$490k
NIGMS NIH HHS R01 GM062261NIH HHS R01-GM062261NIH HHS S10 OD020011NIH HHS S10OD020011NIH HHS S10 OD030275University of Michigan Biosciences InitiativeUniversity of Michigan Cryo-EM FacilityUniversity of Michigan Rackham Predoctoral Fellowship
6 · The paper itself

Abstract

Myo2, a class V myosin motor, is essential for organelle transport in budding yeast. Its association with cargo is regulated by adaptor proteins that mediate both attachment and release. Vac17, a vacuole-specific adaptor, links Myo2 to the vacuole membrane protein Vac8 and plays a key role in assembling and disassembling the Myo2-Vac17-Vac8 complex during vacuole inheritance. Using genetics, cryo-EM, and structure prediction, we find that Vac17 interacts with Myo2 at two distinct sites rather than a single interface. Similarly, the peroxisome adaptor Inp2 engages two separate regions of Myo2, one of which overlaps with a Vac17-binding site. These findings support a "handhold" model, in which cargo adaptors occupy multiple surfaces on the Myo2 tail, which likely enhances motor-cargo associations as well as provide additional regulatory control over motor recruitment.

Indexed as

Myosin Heavy ChainsMyosin Type VOrganellesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVacuolesVesicular Transport ProteinsBinding SitesBiological TransportCryoelectron MicroscopyProtein BindingReceptors, Cell SurfaceMYO2 protein, S cerevisiaeMyosin Heavy ChainsMyosin Type VReceptors, Cell SurfaceSaccharomyces cerevisiae ProteinsVAC17 protein, S cerevisiaeVesicular Transport Proteins

Identifiers

PMID40377475
PMCPMC12083248

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.