Evidence map›Paper›PMID 40376157›Full record

ArticleFrontiers in plant science2025

dCas9-SPO11-1 locally stimulates meiotic recombination in rice.

Léo Herbert, Aurore Vernet, Julien Frouin, Anne Cécile Meunier, Jeremy Di Mattia, Minghui Wang, Gaganpreet K Sidhu, Luc Mathis, Alain Nicolas, Emmanuel Guiderdoni and 1 more

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In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Léo HerbertMeiogenix SA, Paris, France.
Aurore VernetCentre de coopération internationale en recherche agronomique pour le développement (CIRAD), Unité mixte de recherche - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) Institut, Montpellier, France.
Julien FrouinCentre de coopération internationale en recherche agronomique pour le développement (CIRAD), Unité mixte de recherche - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) Institut, Montpellier, France.
Anne Cécile MeunierCentre de coopération internationale en recherche agronomique pour le développement (CIRAD), Unité mixte de recherche - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) Institut, Montpellier, France.
Jeremy Di MattiaIngénierie et Analyse en Génétique Environnementale (IAGE), Montpellier, France.
Minghui WangMeiogenix Inc., Center for Life Science Ventures Cornell University, Ithaca, NY, United States.
Gaganpreet K SidhuMeiogenix Inc., Center for Life Science Ventures Cornell University, Ithaca, NY, United States.
Luc MathisMeiogenix SA, Paris, France.
Alain NicolasMeiogenix SA, Paris, France.
Emmanuel GuiderdoniCentre de coopération internationale en recherche agronomique pour le développement (CIRAD), Unité mixte de recherche - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) Institut, Montpellier, France.
Ian FayosMeiogenix SA, Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Meiotic crossovers shuffle the genetic information transmitted by the gametes. However, the potential to recover all the combinations of the parental alleles remains limited in most organisms, including plants, by the occurrence of only few crossovers Methods: Here, we investigate the use of a dCas9-SPO11-1 fusion and clusters of 11 gRNAs to alter meiotic recombination in two chromosomal regions of a rice hybrid (KalingaIII/Kitaake). To accurately genotype rare recombinants in regions of few kbp, we improved the digital PCR-based pollen-typing method in parallel. Results: Expression of the dCas9-SPO11-1 fusion protein under the ubiquitous Discussion: These results open new avenues to locally stimulate meiotic recombination in crop genomes and paves the way for a future implementation in plant breeding programs.

Indexed as

CRISPR/dCas9meiosisplant breedingpollen typingriceSpo11targeted recombination

Identifiers

PMID40376157
PMCPMC12078263

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.