ArticleJournal of clinical and experimental dentistry2025
Impact of solutions and storage time on the chemical and mechanical properties of human dentin.
Article in Journal of clinical and experimental dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Divergent chemical and mechanical dentin responses to an all-in-one endodontic irrigant.Odontology · 2026Article
- Article
- The antibacterial effect of chlorohexidine, Er:YAG laser and diode laser 980 nm as dental cavity disinfectants of dentine: an in vitro study.European journal of medical research · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Considering the importance of standardization of the pH of control solutions and its impact on the chemical composition and mechanical properties of dentin during storage over a specified period, this study aimed to analyze the pH of control solutions and how it affects the chemical composition and mechanical properties of dentin stored over a given period. Material and Methods: Six control solutions-coconut water, mineral water, distilled water, deionized water, artificial tears, and saline-were kept in a bacteriological incubator at 37°C, with their pH measured using a pH meter (mPA-210 from MS Tecnopon ®) for seven days: T0 (initial), T1 (2 hours), T2 (24 hours), T3 (48 hours), and T4 (7 days). In the second phase, the two solutions with the most stable pH in the first phase were selected and aligned with the critical pH of dentin (pH ≥ 6.5). Human third molars were sectioned and divided into two groups (n=13): distilled water and deionized water. The pH of the solutions, the chemical composition, and the microhardness of the dentin were evaluated by pH meter, Fourier transform infrared spectroscopy (FTIR), and Knoop microhardness (KH), respectively, at the aforementioned time points. The values were analyzed by two-way ANOVA (storage time and solution), followed by the Tukey test. Results: Both solutions presented pH incompatible with the dentin until T2, and the samples presented a 40% reduction in microhardness at T4. Additionally, a reduction in carbonate and an increase in amides were observed in the dentin, indicating changes in the mineral and organic phases. Conclusions: It is concluded that both the solutions and the storage time negatively affect the chemical composition and microhardness of the dentin, highlighting the importance of carefully selecting the control solutions and the storage time in in vitro studies.
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Registered trials
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