Evidence map›Paper›PMID 40374663›Full record

ArticleScientific reports2025

Overexpression MsJAR1 gene increased lateral branches and plant height in alfalfa (Medicago sativa L.).

Rui Dai, Qi Chen, Xiao-Yu Wang, Xiao-Wei Huo, Jian-Wei Li, Jiang-Bo An, Fu-Gui Mi, Feng-Ling Shi, Zhi-Qiang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rui DaiCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Qi ChenCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Xiao-Yu WangCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Xiao-Wei HuoCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Jian-Wei LiCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Jiang-Bo AnCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Fu-Gui MiCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Feng-Ling ShiCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China.
Zhi-Qiang ZhangCollege of Grassland Science, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China. zhangzq1989@imau.edu.cn.

Funding

Alfalfa Molecular Breeding System Construction and Germplasm Creation BR22-11-12Inner Mongolia Autonomous Region Natural Science Foundation 2021SHZR0721Young Science and Technology Talents Support Project NJYT23009
6 · The paper itself

Abstract

Alfalfa (Medicago sativa L.) is an important legume forage known for its high yield, quality, and adaptability. However, due to its cross-pollination and tetraploid inheritance characteristics, biotechnologies such as genetic modification and gene editing are challenging to be utilized in the practice of alfalfa breeding. In this study, a MsJAR1 gene involved in jasmonic acid (JA)pathway was overexpressed into 'Zhongmu No.1' alfalfa, and the effects of MsJAR1 gene on alfalfa development were analyzed. Subcellular localization results indicated that the MsJAR1 protein was located to chloroplast. Compared to wildtype (WT), MsJAR1 overexpressed alfalfa plants displayed smaller leaves, reduced internode distance, increased lateral branches and plant height. Moreover, anatomical analysis revealed that xylem and phloem area decreased in leaves but increased in stems of MsJAR1 overexpressed alfalfa. Except intercellular CO₂, photosynthetic rates, stomatal conductance, and transpiration were significantly higher than in MsJAR1 overexpressed alfalfa. Comparative transcriptomics results showed that 12,046 DEGs were identified between transgenic alfalfa (OE-1 and OE-2) and WT. The DEGs were significantly enriched in ribosome, glycolysis / gluconeogenesis and tricarboxylic acid cycle pathways. Therefore, it is speculated that the MsJAR1 gene affects the growth and development of alfalfa by regulating photosynthesis-related pathways.

Indexed as

Medicago sativaPlant ProteinsChloroplastsCyclopentanesGene Expression ProfilingGene Expression Regulation, PlantOxylipinsPhotosynthesisPlant LeavesPlants, Genetically ModifiedCyclopentanesjasmonic acidOxylipinsPlant ProteinsAlfalfaFunctional analysisMsJAR1 geneTranscriptome

Identifiers

PMID40374663
PMCPMC12081595

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.