Evidence map›Paper›PMID 40374541›Full record

ArticleJournal of microbiology and biotechnology2025

Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma.

Yuanye Ji, Ping Tuo, Shun Zhang, Ting Cai, Liyun Fu, Qinzhi Deng, Houdao Fu, Guosheng Gao, Fajiu Wang, Peng Zhu

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuanye JiDepartment of Medical Laboratory, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Ping TuoDepartment of Medical Laboratory, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Shun ZhangDepartment of Medical Laboratory, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Ting CaiNingbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo City, Zhejiang Province, P.R. China.
Liyun FuDepartment of Hepatology, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Qinzhi DengDepartment of Severe Hepatology, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Houdao FuFu Houdao's Traditional Chinese Medicine Expert Heritage Studio, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Guosheng GaoDepartment of Clinical Laboratory, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Fajiu WangDepartment of Thoracic Surgery, Ningbo NO.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.
Peng ZhuDepartment of Medical Laboratory, Ningbo No.2 Hospital, Ningbo City, Zhejiang Province, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatocellular carcinoma (HCC) is an increasingly prevalent malignant neoplasm on a global scale. Circrna HIPK3 (circHIPK3) has been identified as playing a key role in HCC tumorigenesis and as a novel biomarker. In this study, we aimed at developing a sensitive and accurate method for the detection of circHIPK3 in low load plasma samples using a droplet digital PCR (ddPCR). We designed circHIPK3 gradient primers and probes and optimized the PCR system to improve performance. Then we assessed and compared the linearity and sensitivity of ddPCR and quantitative PCR (qPCR) using the circHIPK3 plasmid DNA as a template. Using these methods, circHIPK3 concentrations were quantitatively determined in 3 cell lines and 40 plasma samples to assess clinical stability. Within the plasmid concentration range of 3

Indexed as

Carcinoma, HepatocellularIntracellular Signaling Peptides and ProteinsLiver NeoplasmsPolymerase Chain ReactionProtein Serine-Threonine KinasesRNA, CircularBiomarkers, TumorCell Line, TumorHumansLimit of DetectionReal-Time Polymerase Chain ReactionSensitivity and SpecificityBiomarkers, TumorHIPK3 protein, humanIntracellular Signaling Peptides and ProteinsProtein Serine-Threonine KinasesRNA, CircularcircHIPK3clinical sample detectionddPCReffect evaluation

Identifiers

PMID40374541
PMCPMC12099620

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.