ReviewACS sensors2025
Paper-Based Vertical Flow Assays for in Vitro Diagnostics and Environmental Monitoring.
Review in ACS sensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Article
- Chemically driven design of N-doped MXene quantum dots for portable sensing and smartphone-integrated platforms.RSC advances · 2026Review
- Deep learning-enhanced dual-mode multiplexed optical sensor for point-of-care diagnostics of cardiovascular diseases.Light, science & applications · 2026Article
- Molecularly Imprinted Polymers as Biomimetic Test Zones in Paper-Based Nucleic Acid Assays-Comparing Vertical and Lateral Flow Formats.Biosensors · 2026Article
- Microfluidic Paper-Based Devices at the Edge of Real Samples: Fabrication Limits, Hybrid Detection, and Perspectives.Micromachines · 2026Article
- NanoArrayPAD-X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care.Biosensors · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Microfluidic paper-based analytical devices (μPADs) are powerful tools for diagnostic and environmental monitoring. Being affordable and portable, μPADs enable rapid detection of small molecules, heavy metals, and biomolecules, thereby decentralizing diagnostics and expanding biosensor accessibility. However, the reliance on two-dimensional fluid flow restricts the utility of conventional μPADs, presenting challenges for applications that require simultaneous multibiomarker analysis from a single sample. Vertical flow paper-based analytical devices (VF-μPADs) overcome this challenge by allowing axial fluid movement through paper stacks, offering several advantages, including (1) enhanced multiplexing capabilities, (2) reduced hook effect for improved accuracy, and (3) shorter assay times. This review provides an overview of VF-μPADs technologies, exploring structural and functional performance trade-offs between VF-μPADs and conventional lateral flow systems. The sensing performance, fabrication methods, and applications in in vitro diagnostics and environmental monitoring are discussed. Furthermore, critical challenges─such as fabrication complexity, data analysis, and scalability─are addressed, along with proposed strategies for mitigating these barriers to facilitate broader adoption. By examining these strengths and challenges, this review presents the potential of VF-μPADs to advance point-of-care testing, particularly in resource-limited settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.