Expanding the Riboglow-FLIM Toolbox with Different Fluorescence Lifetime-Producing RNA Tags.

Zachary Stickelman, Nadia Sarfraz, Morgan K Rice, Ben J Lambeck, Sonja Milkovich, Esther Braselmann

Abstract read

In one paragraph

Article in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Evaluating Linker Architecture in RNA-Detecting Riboglow Probes and Effects on Fluorescence Turn-On.ACS chemical biology · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zachary StickelmanDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.

Nadia SarfrazDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.ORCID 0000-0002-6240-3898

Morgan K RiceDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.

Ben J LambeckDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.

Sonja MilkovichDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.

Esther BraselmannDepartment of Chemistry, Georgetown University, Washington, District of Columbia 20057, United States.ORCID 0000-0002-6877-1333

Funding

Illuminating multiplexed RNA dynamics to interrogate splicing in health and diseaseR35GM150823 · NIGMS · GEORGETOWN UNIVERSITY · PI Esther Braselmann · 2023 to 2026

$1.7M

NIGMS NIH HHS R35 GM150823

6 · The paper itself

Abstract

RNAs are essential elements of biology with subcellular localizations critical for function. Genetically tagging fluorescent RNA reporters to an RNA of interest allows for investigating RNA spatiotemporal dynamics. We previously developed a fluorescence lifetime imaging microscopy (FLIM)-based RNA-tagging platform, Riboglow-FLIM. Here, a genetically encoded RNA tag binds a fluorescent probe, causing an increase in both fluorescence intensity and fluorescence lifetime. Importantly, the Riboglow platform is derived from a bacterial riboswitch RNA family and different riboswitch sequences from nature may build the basis for multiplexing capabilities. We previously observed fluorescence lifetime differences for two RNA tags

Indexed as

Fluorescent DyesOptical ImagingRiboswitchRNAFluorescenceHumansMicroscopy, FluorescenceRNA, BacterialFluorescent DyesRiboswitchRNARNA, Bacterial

Identifiers

PMID40372768

DOI10.1021/acs.biochem.4c00567

PMCPMC12138973

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.