Analysis of Transient Receptor Potential Ion Channels in ME/CFS.

Natalie Eaton-Fitch, Katsuhiko Muraki, Etianne Martini Sasso, Chandi Magawa, Sonya Marshall-Gradisnik

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Natalie Eaton-FitchNational Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, QLD, Australia. n.eaton-fitch@griffith.edu.au.

Katsuhiko MurakiConsortium Health International for ME, Griffith University, Gold Coast, QLD, Australia.

Etianne Martini SassoNational Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, QLD, Australia.

Chandi MagawaNational Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, QLD, Australia.

Sonya Marshall-GradisnikNational Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, QLD, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter provides a comprehensive overview of methodologies currently employed to study ion channels, particularly transient receptor potential melastatin 3 (TRPM3) in the context of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Sample preparation involves the collection of whole blood, separation of peripheral blood mononuclear cells (PBMCs) via density gradient centrifugation, and isolation of natural killer (NK) cells. Protein expression analysis utilizes flow cytometry, liquid chromatography-mass spectrometry (LC-MS), western blotting, and immunofluorescence techniques. Functional analysis focuses on calcium imaging and electrophysiology techniques to investigate ion channel responses to pharmacological stimuli. The authors highlight that some experimental protocols included within this chapter require specialized training and equipment. In order to replicate these protocols extended training is advised, specifically when attempting electrophysiology experimentation. The use of advanced techniques for detailed analysis provides insights into ion channel function and potential implications in the pathomechanism of ME/CFS offering avenues for further research and therapeutic exploration.

Indexed as

Fatigue Syndrome, ChronicTransient Receptor Potential ChannelsChromatography, LiquidFlow CytometryHumansKiller Cells, NaturalLeukocytes, MononuclearMass SpectrometryTransient Receptor Potential ChannelsElectrophysiologyFlow cytometryImmunofluorescenceMyalgic encephalomyelitis/chronic fatigue syndromeNatural killer cellsProtein expressionTransient receptor potential ion channels

Identifiers

PMID40372679

DOI10.1016/B978-0-444-63688-1.00004-5

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.