Evidence map›Paper›PMID 40372678›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry.

Anton Gibson, Thaize Q Chometon, Tanvi Damani, Anna E S Brooks

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anton GibsonLiggins Institute, University of Auckland, Auckland, New Zealand.ORCID 0009-0001-8134-2592
Thaize Q ChometonSchool of Biological Sciences, University of Auckland, Auckland, New Zealand.ORCID 0000-0002-7389-2324
Tanvi DamaniSchool of Biological Sciences, University of Auckland, Auckland, New Zealand.ORCID 0000-0002-7624-3258
Anna E S BrooksLiggins Institute, University of Auckland, Auckland, New Zealand. a.brooks@auckland.ac.nz.ORCID 0000-0003-3551-6982

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immune dysfunction is reported to play a significant role in the etiology of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). To gain an understanding of the underlying immune abnormalities associated with this complex condition, a comprehensive approach for characterizing immune cell subsets and their inferred functional states is essential. We developed a high-dimensional flow cytometry method that enables detailed immunophenotyping of peripheral blood mononuclear cells (PBMCs) from ME/CFS patients. By simultaneously measuring over 40 markers on individual cells within one sample, this approach provides a comprehensive assessment of immune cell subsets, incorporating effector or functional states, to enable assessment of their potential roles in disease pathogenesis.

Indexed as

Fatigue Syndrome, ChronicFlow CytometryImmunophenotypingBiomarkersHumansLeukocytes, MononuclearBiomarkersHigh-dimensional flow cytometryImmune dysfunctionImmunophenotypingPeripheral blood mononuclear cellsSpectral flow cytometry

Identifiers

PMID40372678

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.