ArticleMethods in molecular biology (Clifton, N.J.)2025
Recombinant Topoisomerase 2 Production Using Cultured Human Cell Lines.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Molecular basis of allosteric regulation and pharmaceutical targeting of protein kinase Cβ.Nature communications · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant human Topoisomerase 2 (TOP2) alpha and TOP2 beta proteins are valuable reagents for structural and biochemical analyses. Here, we describe a scalable method for production of high-purity recombinant TOP2 proteins using a HEK293F cell expression system with transient transfection of expression constructs. Human TOP2 is expressed as a fusion with YFP or mCherry, which serves dual purposes as an indicator of protein expression and an affinity tag that enables rapid isolation of TOP2 from cell lysates. Subsequent FPLC polishing of the protein yields milligram-scale yields of high purity and active recombinant TOP2.
Indexed as
Identifiers
40372648What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.