Evidence map›Paper›PMID 40372647›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Kinetoplast DNA Decatenation Assay with Eukaryotic Topoisomerase II.

Addison K O'Brian, Mattalyn R Hardin, Allison J Thomas, Brooke D Latham, Joseph Deweese

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Addison K O'BrianDepartment of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA.
Mattalyn R HardinDepartment of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA.
Allison J ThomasDepartment of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA.
Brooke D LathamDepartment of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA.
Joseph DeweeseDepartment of Biological, Physical, and Human Sciences, Freed-Hardeman University, Henderson, TN, USA. jdeweese@fhu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The interlinking of DNA during replication and other DNA processes is resolved by type II DNA topoisomerases. In humans, topoisomerase IIα is the main enzyme involved in this process using a transient double-stranded DNA break to unlink sister chromatids during and after replication to allow for mitosis. To measure decatenation, a consistent catenated substrate called kinetoplast DNA (kDNA) is commonly used. kDNA is often purified from the trypanosome Crithidia fasciculata. kDNA represents catenated or interlinked circles of DNA. Early work on topoisomerases identified kDNA as a useful substrate for measuring decatenation by DNA topoisomerase II. This protocol will describe a common method for measuring decatenation of kDNA by eukaryotic type II topoisomerases.

Indexed as

DNA, CatenatedDNA, KinetoplastDNA Topoisomerases, Type IIAnimalsHumansDNA, CatenatedDNA, KinetoplastDNA Topoisomerases, Type IIDecatenationGel electrophoresiskDNAKinetoplast DNAMinicirclesTopoisomerase II

Identifiers

PMID40372647

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.