ArticleMethods in molecular biology (Clifton, N.J.)2025
Use of Xenopus Egg Extracts to Study the Effects of Topoisomerase Poisons During Vertebrate DNA Replication.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Topoisomerases unlink chromosomes and relieve topological stress. Topoisomerase "poisons" are widely used chemotherapeutics that stabilize topoisomerase complexes on DNA, leading to cytotoxic DNA breaks and cancer cell killing. It is well established that topoisomerase poisons interfere with DNA replication, which is thought to be a major physiological target of these drugs. However, many questions remain about the mechanisms by which topoisomerase poisons impact DNA replication and the downstream consequences. Here, we describe assays to study topoisomerase poisons during vertebrate DNA replication using Xenopus egg extracts. These approaches allow for replication intermediates formed following poison treatment to be carefully monitored with high temporal resolution.
Indexed as
Identifiers
40372644What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.