Evidence map›Paper›PMID 40372584›Full record

ArticleCell biochemistry and biophysics2025

A Potent Plant-Derived Chitinase: Structural Informatics and Insecticidal Activity against Helicoverpa armigera.

Shah Rukh, Ahmed Akrem, Qamar Saeed, Sohaib Mehmood, Nosherwan Adil, Tazeen Rao, Muhammad Nasir, Uzma Ishaq, Aqal Zaman, Najeeb Ullah and 1 more

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Article in Cell biochemistry and biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shah RukhDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Ahmed AkremInstitute of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Qamar SaeedDepartment of Entomology, Bahauddin Zakariya University, Multan, Pakistan.
Sohaib MehmoodInstitute of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Nosherwan AdilDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Tazeen RaoDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Muhammad NasirDepartment of Entomology, Bahauddin Zakariya University, Multan, Pakistan.
Uzma IshaqInstitute of Botany, Bahauddin Zakariya University, Multan, Pakistan.
Aqal ZamanDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Najeeb UllahDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan. najeebkhattak@bzu.edu.pk.
Mohibullah ShahDepartment of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan. mohib@bzu.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Helicoverpa armigera (cotton bollworm) is a globally distributed lepidopteran pest that causes estimated annual agricultural losses exceeding 5 billion USD. While chemical pesticides remain the primary control strategy, their prolonged use has led to significant environmental contamination, development of widespread insecticide resistance, and non-target organism toxicity. These limitations underscore the critical need for plant-derived biopesticides that offer target specificity, environmental biodegradability, and sustainable pest management solutions without promoting resistance development. Here, we elucidate the insecticidal potential of Nelumbo nucifera Chitinase (NnChi) against the insect H. armigera through structural informatics and in-vivo insecticidal bioassays. SDS-PAGE showed a single band of ~32 kDa, and LC-MS/MS analysis depicted a fragment of 10 amino acids with 100% identity with Family 19 Class I Chitinase of Mangifera indica. NnChi-predicted structure revealed its two domains (ChB D, Cat D) connected through linker region and docking analysis of both these domains with (GlcNAc)

Indexed as

ChitinasesInsecticidesMothsAmino Acid SequenceAnimalsHelicoverpa armigeraLarvaMolecular Docking SimulationMolecular Dynamics SimulationChitinasesInsecticidesHelicoverpa armigeraInsecticidal chitinaseIn-silicoMD simulationNelumbo nuciferaStructure prediction

Identifiers

PMID40372584

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.