Evidence map›Paper›PMID 40372081›Full record

ArticleMicrobiology spectrum2025

Remapping the spatial distribution of neutralizing sites and their immunodominance on the capsid of different topotypes of FMDV serotype O by site-directed competitive ELISA for detection of neutralizing antibodies.

Qiongqiong Zhao, Fengjuan Li, Shulun Huang, Xiangchuan Xing, Ying Sun, Pinghua Li, Huifang Bao, Yuanfang Fu, Pu Sun, Xingwen Bai and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Qiongqiong ZhaoNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China.ORCID 0009-0006-4934-4738
Fengjuan LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Shulun HuangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Xiangchuan XingState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Ying SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Pinghua LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Huifang BaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Yuanfang FuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Pu SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Xingwen BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Hong YuanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Xueqing MaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID 0009-0003-6780-0061
Zhixun ZhaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Jing ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Jian WangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Tao WangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Dong LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Qiang ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Ping QianNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China.
Yimei CaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID 0009-0000-1267-9741
Kun LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID 0000-0001-8307-634X
Xiangmin LiNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei, China.ORCID 0000-0003-1412-5069
Zengjun LuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID 0000-0001-9138-692X

Funding

National Key Research and Development Program of China 2021YFD1800300
6 · The paper itself

Abstract

The foot-and-mouth disease virus (FMDV) serotype O contains at least five neutralizing antigenic sites, yet the structural relationship and antibody abundance remain poorly characterized. This study identifies six distinct neutralizing antigenic sites by evaluating 27 host-derived neutralizing antibodies (NAbs) using competitive enzyme-linked immunosorbent assay (cELISA). These sites include the VP1 G-H loop, VP1 C-terminus, site 2, site 4, site 6, and site 7. Notably, classical sites 1 and 5 were reclassified into the VP1 G-H loop and VP1 C-terminus classes. Sites 2 and 4 align with classical classifications, targeting independent epitopes on VP2 and VP3, respectively. We identified two novel sites: site 6, which involves extensive interactions with the G-H loop, C-terminus of VP1 and VP3, and site 7, which interacts with both VP2 and VP3. Sera from cattle, sheep, and pigs immunized with four serotype O lineages (O/SCGH/2016, O/Mya/98, O/Tibet/99, and O/XJ/2017) were used to evaluate the immunodominance of these sites. NAb responses favored site 4 for O/SCGH/2016 and the VP1 G-H loop for O/XJ/2017. Immunization effectiveness varied by strains and host species: O/XJ/2017 and O/Tibet/99 were effective in sheep, while O/Mya/98 showed reduced efficacy; O/Tibet/99 showed good immunogenicity in pigs. No significant differences were observed in cattle. There is a strong correlation (r = 0.8693) between NAb levels at site 6 and virus neutralization tests, suggesting its potential for use in alternative testing methods. This study describes the spatial distribution of neutralizing sites and highlights strain-specific immunodominant epitopes and differential antibody responses across species, providing valuable insights for FMD prevention and control. IMPORTANCE: The antigenic structure of the foot-and-mouth disease virus (FMDV) serotype O is complex, and the immunodominant epitopes among different lineages remain poorly understood. This study classified the capsid surface epitopes into six distinct antigenic sites utilizing 27 neutralizing antibodies (NAbs) by paired competitive ELISAs (cELISAs). High-affinity NAbs were selected for site-directed cELISAs to assess antibody abundance in serum from cattle, sheep, and pigs vaccinated with various inactivated FMDV serotype O vaccines. Additionally, liquid-phase blocking ELISA (LPBE) and virus neutralization test (VNT) were employed to measure total antibody and NAb titers. Results indicated that immunodominant sites vary among different strains, with each strain exhibiting different immunogenicity across the three animal species. Notably, antibody titers from NAb pO18-10, targeting site 6 on VP1 and VP3, correlated strongly with VNT results. This study provides comprehensive insights into the antigenic structure of FMDV serotype O and lays the groundwork for developing new methods to detect NAbs.

Indexed as

Antibodies, NeutralizingAntibodies, ViralCapsidCapsid ProteinsFoot-and-Mouth Disease VirusImmunodominant EpitopesAnimalsAntigens, ViralCattleEnzyme-Linked Immunosorbent AssayFoot-and-Mouth DiseaseSerogroupSheepSwineAntibodies, NeutralizingAntibodies, ViralAntigens, ViralCapsid ProteinsImmunodominant Epitopescompetitive ELISAfoot-and-mouth disease virusneutralizing antibodyneutralizing antigenic sitesserotype O

Identifiers

PMID40372081
PMCPMC12131818

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.