Evidence map›Paper›PMID 40370406›Full record

ArticleFrontiers in cellular and infection microbiology2025

Vγ9Vδ2 T cells expanded with vitamin C combined with HMBPP

Shuyan Liu, Guohui Xiao, Wanxin Liang, Su Zhang, Juan Liang, Teng Pan, Shaoxiang Lin, Xiuju Liu, Zhenwen Zhou, Guoliang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Shuyan LiuNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
Guohui XiaoNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
Wanxin LiangNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
Su ZhangNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
Juan LiangNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.
Teng PanLonggang Maternity and Child Institute of Shantou University Medical College, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, China.
Shaoxiang LinLonggang Maternity and Child Institute of Shantou University Medical College, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, China.
Xiuju LiuLonggang Maternity and Child Institute of Shantou University Medical College, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, China.
Zhenwen ZhouLonggang Maternity and Child Institute of Shantou University Medical College, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, China.
Guoliang ZhangNational Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Methods: Venous blood was collected from healthy individuals, and PBMCs were subsequently isolated. In vitro, Vγ9Vδ2 T cells were selectively expanded with HMBPP, rIL-2, and VC. Flow cytometry was utilized to analyze the purities and phenotypes of Vγ9Vδ2 T cells, while cell counts were performed to determine the total number of viable cells. Magnetic bead sorting was employed to purify Vγ9Vδ2 T cells. Results: VC (70 μM) significantly enhances the expansion of Vγ9Vδ2 T cells within PBMCs during primary HMBPP activation in the presence of rIL-2, with higher induction rates and total cell proliferation. By day 14 of induction, Vγ9Vδ2 T cells expanded with HMBPP, VC, and rIL-2 exhibited the central memory (10-20%) and the effector memory phenotypes (75-90%). Furthermore, these expanded T cells effectively inhibited the growth of intracellular virulent Conclusion: This study demonstrates that VC enhances the proliferative expansion of Vγ9Vδ2 T cells in PBMCs primarily stimulated with HMBPP and rIL-2. The expanded Vγ9Vδ2 T cells are capable of effectively inhibiting the growth of virulent H37Rv strain, likely through the secretion of TNF-α and IFN-γ. These findings provide a novel direction for tuberculosis treatment research.

Indexed as

Ascorbic AcidMycobacterium tuberculosisT-LymphocytesCell ProliferationCoculture TechniquesCytokinesHumansInterferon-gammaInterleukin-10Interleukin-2Leukocytes, MononuclearLymphocyte ActivationMacrophagesOrganophosphatesReceptors, Antigen, T-Cell, gamma-deltaTHP-1 Cells4-hydroxy-3-methyl-2-butenyl diphosphateAscorbic AcidCytokinesInterferon-gammaInterleukin-10Interleukin-2OrganophosphatesReceptors, Antigen, T-Cell, gamma-deltaeffector functionHMBPPinduction rateinhibitionMycobacterium tuberculosisvitamin CVγ9Vδ2 T cells

Identifiers

PMID40370406
PMCPMC12075132

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.