ArticleNature nanotechnology2025
Nanopore-based enzyme-linked immunosorbent assay for cancer biomarker detection.
Article in Nature nanotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Orthogonal nanopores cross-validation for multiplex single-molecule profiling.Chemical science · 2026Article
- Renal Clearable Luminogenic Reporter for Ultrasensitive Influenza Virus Imaging and Efficient Antiviral Therapies Monitoring in Living Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing.Angewandte Chemie (International ed. in English) · 2026Article
- Harnessing intrinsic electron-blocking effect of wide-bandgap BiCoO₃ nanosheets for ultrasensitive label-free impedimetric immunodetection of carcinoembryonic antigen.Mikrochimica acta · 2026Article
- Tumor organoids as a revolutionary platform for advancing cancer nanomedicine.Molecular cancer · 2026Review
- Enzyme-Integrated Hydrogels for Advanced Biological Applications.Polymer science & technology (Washington, D.C.) · 2026Review
- Electrochemical tyrosine-click bioconjugation enables multiplexed cytokine sensing and immunoprofiling in native serum.Nature communications · 2026Article
- A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Actinomycin in Human Serum.ACS measurement science au · 2026Article
- Nanopore Label-Free Monitoring of B-DNA and Z-DNA Conformational Transition in Real Time.ACS nano · 2026Article
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
- De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs.ACS nano · 2025Article
- Enzyme-assisted nanopore sensor for sensitive and multiplexed detection of microRNAs.Journal of nanobiotechnology · 2025Article
- Nanopore-based sensing for biomarker detection: from fundamental principles to translational diagnostics.Journal of nanobiotechnology · 2025Review
- The Potential of Nanopore Technologies in Peptide and Protein Sensing for Biomarker Detection.Biosensors · 2025Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Enzyme-linked immunosorbent assay (ELISA) has been widely used in cancer diagnostics due to its specificity, sensitivity and high throughput. However, conventional ELISA is semiquantitative and has an insufficiently low detection limit for applications requiring ultrahigh sensitivity. In this study, we developed an α-hemolysin-nanopore-based ELISA for detecting cancer biomarkers. After forming the immuno-sandwich complex, peptide probes carrying enzymatic cleavage sites are introduced, where they interact with enzymes conjugated to the detection antibodies within the complex. These probes generate distinct current signatures when translocated through the nanopore after enzymatic cleavage, enabling precise biomarker quantification. This approach offers a low detection limit of up to 0.03 fg ml
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Registered trials
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