Evidence map›Paper›PMID 40369262›Full record

ArticleJournal of assisted reproduction and genetics2025

Biological effects of femtosecond and millisecond lasers application for assisted hatching in mouse embryos.

Maxim A Filatov, Marina V Kubekina, Anna V Tvorogova, Leonid A Ilchuk, Alexander D Zhuravlev, Elizaveta A Sazonova, Anna D Ivanova, Yuliya Yu Silaeva, Dmitry S Sitnikov

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In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. The effect of laser-assisted incubation on bovineFrontiers in veterinary science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maxim A FilatovInstitute of Gene Biology of the Russian Academy of Sciences, Vavilova St. 34/5, Moscow, 119334, Russian Federation. maxfilat@yandex.ru.ORCID http://orcid.org/0000-0003-0323-5967
Marina V KubekinaInstitute of Gene Biology of the Russian Academy of Sciences, Vavilova St. 34/5, Moscow, 119334, Russian Federation.ORCID http://orcid.org/0000-0002-8834-1111
Anna V TvorogovaInstitute of Gene Biology of the Russian Academy of Sciences, Vavilova St. 34/5, Moscow, 119334, Russian Federation.ORCID http://orcid.org/0000-0002-8205-1914
Leonid A IlchukInstitute of Gene Biology of the Russian Academy of Sciences, Vavilova St. 34/5, Moscow, 119334, Russian Federation.ORCID http://orcid.org/0000-0002-0157-2102
Alexander D ZhuravlevLaboratory of Cellular and Molecular Pathology of Cardiovascular System, Federal State Budgetary Scientific Institution "Petrovsky National Research Centre of Surgery", 3 Tsyurupa Street, Moscow, 117418, Russian Federation.ORCID http://orcid.org/0000-0002-0451-2594
Elizaveta A SazonovaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 12-1, Moscow, 119234, Russian Federation.ORCID http://orcid.org/0009-0009-6007-3897
Anna D IvanovaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 12-1, Moscow, 119234, Russian Federation.ORCID http://orcid.org/0000-0003-0988-3009
Yuliya Yu SilaevaInstitute of Gene Biology of the Russian Academy of Sciences, Vavilova St. 34/5, Moscow, 119334, Russian Federation.ORCID http://orcid.org/0000-0003-2070-9001
Dmitry S SitnikovJoint Institute for High Temperatures of the Russian Academy of Sciences, Izhorskaya St. 13 Bd.2, Moscow, 125412, Russian Federation.ORCID http://orcid.org/0000-0002-5283-5283

Funding

Ministry of Science and Higher Education of the Russian Federation 075-15-2019-1661Russian Science Foundation 23-19-00424
6 · The paper itself

Abstract

purposeLaser-assisted hatching (LAH) is a typical procedure in ART. It is used when embryos are unable to hatch. To reduce the potential damage to embryos caused by millisecond laser typically used to create zona pellucida openings, we have developed a protocol for femtosecond laser microsurgery. The aim of this study was to investigate the possible effects of the femtosecond laser, as opposed to the millisecond dissector used in LAH.

methodsZygotes obtained from hormonally stimulated wild-type mice were cultured in vitro until blastocyst stage. Two types of lasers were used to perform LAH: femtosecond and millisecond. The femtosecond laser system used in this study was a TETA ytterbium laser. As a millisecond laser, we used the Octax Laser Shot dissector (pulse duration 5 and 10ms).

resultsTo analyze whether the femtosecond laser could be dangerous for embryos, we evaluated the presence of reactive oxygen species (ROS) and heat-shock proteins (HSP). We found no differences in the production of ROS and in the synthesis of HSP at both the protein and mRNA levels between the negative control and the femtosecond LAH groups, whereas ROS levels in the millisecond LAH group of embryos were close to those observed in the positive control group. Millisecond laser-treated embryos collapsed, while embryos from the femtosecond LAH group did not exhibit such morphological changes.

conclusionsApplication of femtosecond laser for LAH procedure is effective and does not lead to the production of HSP and ROS in embryos.

Indexed as

Embryo, MammalianLasersReproductive Techniques, AssistedAnimalsBlastocystEmbryonic DevelopmentFemaleHeat-Shock ProteinsMiceReactive Oxygen SpeciesZona PellucidaZygoteHeat-Shock ProteinsReactive Oxygen SpeciesBlastocystFemtosecond laserHeat shock proteinsLaser assisted hatchingMiceReactive oxygen species

Identifiers

PMID40369262
PMCPMC12356777

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.