Evidence map›Paper›PMID 40369096›Full record

ArticleScientific reports2025

A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli.

Bingyu Xue, Huanrong Zhang, Xia Yan, Xiaoyan Su, Yuling Zhou, Junjin Xie, Sheng Li, Yang Zhou, Lin Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Deciphering the Implications ofPathogens (Basel, Switzerland) · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bingyu XueKey Laboratory of Veterinary Medicine in Universities of Sichuan Province, Southwest Minzu University, Chengdu, 610041, Sichuan, China.
Huanrong ZhangKey Laboratory of Veterinary Medicine in Universities of Sichuan Province, Southwest Minzu University, Chengdu, 610041, Sichuan, China.
Xia YanSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China.
Xiaoyan SuSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China.
Yuling ZhouKey Laboratory of Veterinary Medicine in Universities of Sichuan Province, Southwest Minzu University, Chengdu, 610041, Sichuan, China.
Junjin XieSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China.
Sheng LiSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China.
Yang ZhouSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China.
Lin LiSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, China. lilin_yx@163.com.

Funding

Chengdu Research Base of Giant Panda Breeding 2024CPB-B11
6 · The paper itself

Abstract

Diarrheagenic Escherichia coli (DEC) are strains of Escherichia coli (E. coli) that can induce diarrhea symptoms in the host, as well as cause disease through contaminated food. To accurately and efficiently identify the five DEC types, this study retrieved the corresponding gene sequences from NCBI based on the virulence genes specified in Chinese national standards: invE, stx1, stx2, sth, stp, lt, aggR, astA, pic, bfpB, and escV. Probes and primers were designed for the conserved regions of these genes, and the amplification system, temperature, and other parameters were optimized. A TaqMan

Indexed as

DiarrheaEscherichia coliEscherichia coli InfectionsReal-Time Polymerase Chain ReactionDNA, BacterialEscherichia coli ProteinsHumansLimit of DetectionSensitivity and SpecificityDNA, BacterialEscherichia coli ProteinsDECDetectionqPCRTaqMan probe

Identifiers

PMID40369096
PMCPMC12078567

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.