Evidence map›Paper›PMID 40368982›Full record

ArticleScientific reports2025

Integration of GWAS and transcriptome and haplotype analyses to identify QTNs and candidate genes controlling oil content in soybean seeds.

Kaixin Zhang, Bo Hu, Wenhao Wang, Wen-Xia Li, Hailong Ning

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kaixin ZhangNortheast Agricultural University, Harbin, 150030, China.
Bo HuNortheast Agricultural University, Harbin, 150030, China.
Wenhao WangNortheast Agricultural University, Harbin, 150030, China.
Wen-Xia LiNortheast Agricultural University, Harbin, 150030, China. liwenxianeau@126.com.
Hailong NingNortheast Agricultural University, Harbin, 150030, China. ninghailongneau@126.com.

Funding

grant from Opening Competition Mechanism to Select the Best Candidates Project of Heilongjiang Province Department of Science and Technology 2023ZXJ02B02National Natural Science Foundation of China 32201874
6 · The paper itself

Abstract

The oil content (OC) of soybean is a critical trait with important applications in the development of both food and industrial products. Consequently, enhancing OC has consistently remained a significant objective in soybean breeding programs. In this research, a four-way recombinant inbred line (FW-RIL) population containing 144 lines developed from the cross (Kenfeng14 × Kenfeng15) × (Kenfeng19 × Heinong48) was planted in ten environments to investigate the phenotypic data for OC in seeds. On the basis of the genotype data for 109,676 nonredundant single-nucleotide polymorphism (SNP) markers obtained in previous studies, important quantitative trait nucleotides (QTNs) controlling OC in soybean were identified via five multilocus genome-wide association study (GWAS) methods. A total of 54 significant QTNs were detected by the five methods, including 21 QTNs identified by multiple methods and 2 QTNs detected in two environments. By combining pathway analysis, transcriptome sequencing and gene annotation information, two candidate genes, Glyma.18G027100 and Glyma.03G021800, which are likely related to oil synthesis and metabolism in soybean seeds, were identified, and they were then verified via haplotype analysis. Additionally, 12 of the 23 important QTNs were found for the first time in this study. These results not only contribute to the understanding of the genetic control of OC in soybean seeds but are also helpful in marker-assisted selection (MAS) for breeding high-oil varieties of soybean.

Indexed as

Genome-Wide Association StudyGlycine maxHaplotypesQuantitative Trait LociSeedsTranscriptomeGenes, PlantGenotypePhenotypePlant BreedingPolymorphism, Single NucleotideCandidate geneMultilocus GWASOil contentQTNsSoybean

Identifiers

PMID40368982
PMCPMC12078465

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.