Evidence map›Paper›PMID 40368969›Full record

ArticleScientific reports2025

Transient transfection using 222 nm far UV-C irradiation.

Mane Nishimura, Yuki Shimizu, Tomohiro Fujii, Yu Okada, Takeshi Yamamoto, Yoshimasa Ogawa, Toru Koi, Yutaka Suehiro, Takahiro Yamasaki, Jun Nishikawa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mane Nishimura *Faculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Yuki Shimizu *Faculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Tomohiro FujiiFaculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Yu OkadaFaculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Takeshi YamamotoFaculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Yoshimasa OgawaUshio Inc., Chiyoda-ku, Tokyo, Japan.
Toru KoiUshio Inc., Chiyoda-ku, Tokyo, Japan.
Yutaka SuehiroDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Takahiro YamasakiDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Jun NishikawaFaculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan. junnis@yamaguchi-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ultraviolet (UV) light is never used for gene transfer because it damages DNA and harms cellular and plasmid DNA. A light source selectively radiating Far UV-C (F-UV) light causes less DNA damage. We investigated potential introduction of plasmids into cells by irradiating them with F-UV light. COS-7 and CHO-K1 cells were irradiated with 222 nm F-UV light. Then, DNA solution containing green fluorescent protein (EGFP) plasmid was added to the culture and incubated (37 °C, 5% CO

Indexed as

TransfectionUltraviolet RaysAnimalsChlorocebus aethiopsCHO CellsCOS CellsCricetinaeCricetulusGreen Fluorescent ProteinsMicroscopy, FluorescencePlasmidsenhanced green fluorescent proteinGreen Fluorescent Proteins222 nmDeep-ultravioletDNA damageFar UV-CTransfection

Identifiers

PMID40368969
PMCPMC12078505

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.