Evidence map›Paper›PMID 40366593›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission.

Karelia Velázquez, Mari Carmen Vives, Susana Ruiz-Ruiz, José Guerri, Daniel Ventimilla, Javier Terol, Miguel A Pérez-Amador, Manuel Talón, Francisco R Tadeo

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Karelia VelázquezCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Mari Carmen VivesCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain. vives_mac@gva.es.
Susana Ruiz-RuizCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
José GuerriCentro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Daniel VentimillaCentro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Javier TerolCentro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Miguel A Pérez-AmadorInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
Manuel TalónCentro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Francisco R TadeoCentro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain. tadeo_fra@gva.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus-induced gene silencing (VIGS) is a powerful and cost-effective biotechnological tool for studying gene function in plants. It allows for rapid downregulation of gene expression without the need for tissue culture or genetic transformation. While VIGS is transient and may pose challenges for certain developmental genes, it has been successfully used to study organ abscission. Using a VIGS protocol based on Citrus leaf blotch virus (CLBV) viral vectors, we were able to characterize the regulatory role of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-like genes and their potential HAESA (HAE)-like receptors in the process of corolla abscission in Nicotiana benthamiana flowers.

Indexed as

FlowersGene SilencingGenes, PlantNicotianaPlant VirusesGene Expression Regulation, PlantGenetic VectorsPlant ProteinsPlant ProteinsCitrus leaf blotch virus (CLBV)Corolla abscissionGene functionNicotiana benthamianaVirus-induced gene silencing (VIGS)

Identifiers

PMID40366593

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.