ArticleMethods in molecular biology (Clifton, N.J.)2025
From the Field to the Lab: Establishing Different Abscission Potentials in Apple Fruitlets for Transcriptomic and Functional Studies.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physiological studies of the abscission process in non-model species require a profound knowledge of both the specific characteristics of the crop/variety and the multiple factors that are involved in the induction of the process. The main problem deals with the predictability of the destiny of the abscising organ, that is fundamental in comparative physiological studies aimed at identifying the causal factors preceding the activation of the abscission zones (AZs) and the detachment of the organ. Relevant progress has been made in the last decades regarding the abscission of the apple (Malus domestica L. Borkh) fruitlets, which were shown to be a good model system for these kinds of studies. The present method summarizes the most relevant knowledge regarding the following: (1) the establishment of differentially abscising fruitlet populations in apple; (2) the sampling techniques to adopt for successful collections of suitable samples; (3) a reference protocol for the isolation of total RNA from apple fruitlets' tissues, and (4) the main bioinformatic steps to analyze RNA-Seq data. This method may represent a reliable knowledge base for developing of similar approaches in other crops in future.
Indexed as
Identifiers
40366592What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.