Evidence map›Paper›PMID 40366591›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Polar Lipid Extraction and Analysis During Conifer Needle Abscission.

Mason T MacDonald, Chijioke U Emenike

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mason T MacDonaldDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS, Canada. mason.macdonald@dal.ca.
Chijioke U EmenikeDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracting and analyzing polar lipids during conifer needle abscission is crucial for comprehending the associated lipid dynamics. A combination of polar and nonpolar solvents for lipid extraction is actively used in lipid analysis during conifer needle abscission. Conifer needle tissue is particularly difficult to work on for several reasons, which must be considered during sample preparation and lipid extraction. Further, conditions like in situ tissue harvest, postharvest tissue harvest, and extraction protocol influence the analysis of target lipids in the plant. The use of electrospray ionization tandem mass spectrometry (ESI-MS/MS) facilitates the simultaneous and thorough analysis of many polar lipids in conifer tissue samples.

Indexed as

LipidsPlant LeavesTracheophytaSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryLipidsChloroform:methanolConiferFatty acidGalactolipidMass spectrometryPhospholipidPlant lipids

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.