Evidence map›Paper›PMID 40366589›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Staining Methods for Visualization of Cellular Damage During Petal Abscission.

Yuki Furuta, Nobutoshi Yamaguchi, Toshiro Ito

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yuki FurutaDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
Nobutoshi YamaguchiDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan. nobuy@bs.naist.jp.
Toshiro ItoDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Petal abscission involves cell death and reactive oxygen species (ROS) accumulation in the cells at the base of petals. Visualizing changes in the properties of these cells is crucial for analyzing and understanding petal abscission, a trait with important implications, especially for ornamental flower crops. This protocol describes the guidelines, experimental setups, and conditions for visualizing cell death by trypan blue staining and ROS accumulation by 3,3'-diaminobenzidine (DAB) staining in petals. Additionally, it provides instructions for staining and sectioning the entire Arabidopsis thaliana flower to give an improved view of the cells crucial for abscission. This protocol can be used to study the mechanism of petal abscission, including temporal changes at the base of petals during abscission and comparisons with mutants. Although Arabidopsis thaliana and cherry (Prunus sp.) blossoms are used as examples here, this protocol can easily be adapted for other plant species.

Indexed as

ArabidopsisFlowersStaining and Labeling3,3'-DiaminobenzidineCell DeathReactive Oxygen Species3,3'-DiaminobenzidineReactive Oxygen SpeciesArabidopsis thalianaCell deathCherry blossomsDAB stainingPetal abscissionReactive oxygen speciesSenescenceTrypan blue staining

Identifiers

PMID40366589

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.