Evidence map›Paper›PMID 40366160›Full record

ArticlemBio2025

Dual oxic-anoxic co-culture enables direct study of anaerobe-host interactions at the airway epithelial interface.

Patrick J Moore, Kayla Hoffman, Sara Ahmed, Joshua R Fletcher, Talia D Wiggen, Sarah K Lucas, Sabrina J Arif, Adam J Gilbertsen, Leslie A Kent, Jessica K Fiege and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Patrick J MooreDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Kayla HoffmanDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Sara AhmedDepartment of Microbiology and Immunology, SUNY at Buffalo, Buffalo, New York, USA.
Joshua R FletcherDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0002-0723-3195
Talia D WiggenDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Sarah K LucasDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0003-1676-5801
Sabrina J ArifDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Adam J GilbertsenDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Leslie A KentDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Jessica K FiegeDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Ryan A LangloisDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0002-0515-571X
Scott M O'GradyDepartment of Animal Science, University of Minnesota, Saint Paul, Minnesota, USA.
Ryan C HunterDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.ORCID 0000-0003-3841-1676

Funding

The role of anaerobic microbiota in cystic fibrosis airway disease trajectoryR01AI177613 · NIAID · UNIVERSITY OF MINNESOTA · PI Ryan Coulson Hunter · 2023 to 2026
$2.5M
Cystic Fibrosis Foundation FLETC22F0Cystic Fibrosis Foundation MOORE20F0Esselen CF Trust FundNational Institute of Allergy and Infectious Diseases R01AI177613NHLBI NIH HHS HL136919NIAID NIH HHS R01 AI177613NIDCR NIH HHS T90DE0227232
6 · The paper itself

Abstract

Strict and facultative anaerobic bacteria are widely associated with both acute and chronic airway diseases. However, their potential role(s) in disease pathophysiology remains poorly understood due to inherent limitations of existing laboratory models and conflicting oxygen demands between anaerobes and host cells. To address these limitations, here, we describe a dual oxic-anoxic culture (DOAC) approach that maintains an oxygen-limited microenvironment at the apical epithelial interface while host cells are oxygenated basolaterally. This platform enables epithelial-anaerobe co-culture for ~48 h, and we demonstrate its utility by evaluating reciprocal interactions between the oxygen-sensitive anaerobic bacterium,

Indexed as

Bacteria, AnaerobicEpithelial CellsFusobacterium nucleatumHost-Pathogen InteractionsAnaerobiosisCoculture TechniquesFusobacterium InfectionsHumansOxygenOxygenairway epitheliumFusobacterium nucleatumRNAseqsingle-cell RNA sequencing

Identifiers

PMID40366160
PMCPMC12077211

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.