ArticleFrontiers in plant science2025
Assessing
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Biometeorological regulation of male and female fertility traits in banana (Musa spp.) across contrasting flowering environments.Scientific reports · 2026Article
- Imaging of Molecular and Developmental Responses to Abiotic Stresses in Reproductive Tissues.Physiologia plantarumReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Pollen plays a critical role in transgene flow between non-transgenic plants, influencing gene dispersal and environmental risk considerations. Jatropha ( Methods: Pollen viability of X8#34 transgenic and non-transgenic Jatropha was assessed using various staining techniques. An optimized double-staining technique with Fluorescein Diacetate (FDA) and Propidium Iodide (PI) was developed, effectively differentiated viable pollen (green fluorescence) from non-viable (red fluorescence). The effects of temperatures (18°C, 28°C, 30°C 35°C, 40°C and 45°C) and UV-B irradiation (3 to 15 W/m2) on pollen viability ware examined. Additionally, viability was assessed under field-relevant conditions, including sunny and cloudy/shady atmospheric environments. Results: A significant reduction in pollen viability was observed under extreme temperature and UV-B stress. Among different temperatures tested, high temperatures (35°C, 40°C and 45°C) led to a significant decline in pollen viability, with notable differences emerging from 15 min of incubation. Additionally, exposure to high-intensity UV-B irradiation (12 W/m2 and 15 W/m2) significantly reduced the pollen viability. Under a field relevant sunny condition, viability dropped to 19% in transgenic and 16% in non-transgenic after 45 min incubation and complete loss was recorded in 90 min in both genotypes. In cloudy/shady conditions, over 97% of pollen lost viability in 240 min incubation. Statistical analysis confirmed no significant difference is pollen viability between X8#34 and non-transgenic Jatropha across all tested conditions. Discussion: This study provides the first comprehensive assessment of pollen viability in transgenic and non-transgenic Jatropha. The findings highlight the significant influence of environmental factors, particularly temperature and UV-B exposure, on pollen longevity. The optimized double staining technique (FDA + PI) provides a reliable method for assessing pollen viability and may be useful in environmental risk evaluations of transgenic Jatropha. Given the rapid decline in pollen viability under field-relevant conditions, the likelihood of transgene flow via pollen appears limited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.