Evidence map›Paper›PMID 40364985›Full record

ArticleBio-protocol2025

TurboID Labeling and Analysis of Proteins in the Primary Cilium.

Xiaoliang Liu, Xuecai Ge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Xiaoliang LiuDepartment of Molecular and Cell Biology, University of California, Merced, Merced California, CA, USA.
Xuecai GeDepartment of Molecular and Cell Biology, University of California, Merced, Merced California, CA, USA.

Funding

Regulation of the Hedgehog Pathway by new cilium proteins in Development and DiseaseR01GM143276 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GE, XUECAI · 2022 to 2025
$1.5M
Target PDE4D to inhibit the Hedgehog pathwayR15CA235749 · NCI · UNIVERSITY OF CALIFORNIA, MERCED · PI GE, XUECAI · 2019 to 2022
$795k
Mechanistic study of Hedgehog signaling in the ciliumR21CA274595 · NCI · UNIVERSITY OF CALIFORNIA, MERCED · PI GE, XUECAI · 2022 to 2022
$385k
NCI NIH HHS R15 CA235749NCI NIH HHS R21 CA274595NIGMS NIH HHS R01 GM143276
6 · The paper itself

Abstract

Known as the cell's antenna and signaling hub, the primary cilium is a hair-like organelle with a few micrometers in length and 200-300 nm in diameter. Due to the small size of the primary cilium, it is technically challenging to profile ciliary proteins from mammalian cells. Traditional methods, such as physical isolation of cilia, are susceptible to contamination from other cellular components. Other proximity-based labeling methods via APEX or BioID have been used to map ciliary proteins. However, these approaches have their inherent limitations, including the use of toxic reagents like H

Indexed as

BiotinylationMass spectrometryPrimary ciliumProteomicsProximity labelingStable cell lineTurboID

Identifiers

PMID40364985
PMCPMC12067308

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.