Evidence map›Paper›PMID 40362302›Full record

ArticleInternational journal of molecular sciences2025

Cholesterol and SREBP2 Dynamics During Spermatogenesis Stages in Rabbits: Effects of High-Fat Diet and Protective Role of Extra Virgin Olive Oil.

María Virginia Avena, Abi Karenina Funes, María Ángeles Monclus, Paola Vanina Boarelli, Luis Fernando Barbisan, M Rosa Bernal-López, Ricardo Gómez-Huelgas, Tania Estefania Saez Lancellotti, Miguel Walter Fornés

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

María Virginia AvenaAndrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina.ORCID 0000-0003-0752-7713
Abi Karenina FunesAndrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina.
María Ángeles MonclusAndrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina.
Paola Vanina BoarelliMetabolic Diseases Laboratory (LEM), Juan Agustín Maza University, Mendoza M5519, Argentina.ORCID 0000-0001-6709-6447
Luis Fernando BarbisanBiosciences Institute, Department of Structural and Functional Biology, São Paulo State University (UNESP), Botucatu 18610-034, SP, Brazil.ORCID 0000-0002-2180-1814
M Rosa Bernal-LópezInternal Medicine Department, Regional University Hospital of Málaga, Málaga Biomedical Research Institute (IBIMA), 29009 Málaga, Spain.
Ricardo Gómez-HuelgasInternal Medicine Department, Regional University Hospital of Málaga, Málaga Biomedical Research Institute (IBIMA), 29009 Málaga, Spain.
Tania Estefania Saez LancellottiAndrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina.ORCID 0000-0001-8830-6482
Miguel Walter FornésAndrological Research Laboratory of Mendoza (LIAM), IHEM, National University of Cuyo, CONICET, Mendoza M5502JMA, Argentina.ORCID 0000-0002-8634-1396

Funding

Consejo Nacional de Investigaciones Científicas y Técnicas PIP No.: 1236 and PUE No.: 0025Ministerio de Ciencia, Tecnología e Innovación MINCyT, PICT No.: 1236Universidad Nacional de Cuyo Grant: No.: J018-T1
6 · The paper itself

Abstract

High-fat diets (HFDs) have been found to compromise male fertility, with cholesterol dysregulation being a key factor. Sterol regulatory element-binding protein 2 (SREBP2) is a crucial transcription factor that regulates cholesterol biosynthesis and uptake, playing an essential role in maintaining cholesterol homeostasis in the testes. This study investigated the dynamics of SREBP2 and cholesterol levels during rabbit spermatogenesis under HFD conditions. Our findings reveal that SREBP2 expression fluctuates throughout the seminiferous epithelium cycle. However, HFDs induce stage-specific disruptions in cholesterol balance, leading to sperm with increased membrane cholesterol, a reduced sperm count in semen analysis, impaired motility, abnormal morphology, and decreased functionality. In the control group, SREBP2 expression patterns underscored its critical role in normal spermatogenesis. Interestingly, supplementation with extra virgin olive oil (EVOO) reversed the negative effects of HFD, normalizing SREBP2 expression and cholesterol content, which improved sperm quality. These findings emphasize the importance of stage-specific analysis in understanding how dietary fat impacts male fertility and suggest that EVOO may serve as a potential nutritional intervention to protect reproductive health.

Indexed as

CholesterolDiet, High-FatOlive OilSpermatogenesisSterol Regulatory Element Binding Protein 2AnimalsMaleRabbitsSpermatozoaSperm MotilityTestisCholesterolOlive OilSterol Regulatory Element Binding Protein 2cholesterolextra virgin olive oil (EVOO)high-fat dietrabbitsspermatogenesisSREBP2stages of the seminiferous epithelium cycle

Identifiers

PMID40362302
PMCPMC12071441

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.