Evidence map›Paper›PMID 40362275›Full record

ArticleInternational journal of molecular sciences2025

Identification and Exploration of Pyroptosis-Related Genes in Macrophage Cells Reveal Necrotizing Enterocolitis Heterogeneity Through Single-Cell and Bulk-Sequencing.

Peipei Zhang, Ying Li, Panpan Xu, Peicen Zou, Sihan Sheng, Ruiqi Xiao, Pu Xu, Ying Chen, Yue Du, Lishuang Ma and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Peipei ZhangCapital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.ORCID 0000-0002-1878-3803
Ying LiDepartment of Neonatology, Capital Center for Children's Health, Capital Medical University, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China.
Panpan XuCapital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Peicen ZouCapital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Sihan ShengCapital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Ruiqi XiaoCapital Institute of Pediatrics, Beijing 100020, China.
Pu XuCapital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Ying ChenDepartment of Neonatology, Capital Center for Children's Health, Capital Medical University, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China.
Yue DuDepartment of Neonatology, Capital Center for Children's Health, Capital Medical University, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China.
Lishuang MaDepartment of Neonatology Surgery, Capital Center for Children's Health, Capital Medical University, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China.
Yajuan WangDepartment of Neonatology, Capital Center for Children's Health, Capital Medical University, No. 2 Yabao Road, Chaoyang District, Beijing 100020, China.

Funding

high-level Public Health Technical Personnel Construction Project of Beijing 367 Municipal Health Commission (code: Principal investigator-03-02)Research Foundation of Capital Institute of Pediatrics LCYJ-2025-38
6 · The paper itself

Abstract

Necrotizing enterocolitis (NEC) is an acute intestine dysfunction intestinal disorder characterized by inflammation and cell death, including pyroptosis. Previous studies have implicated pyroptosis, particularly via NLRP3 inflammatory activation, and contribute to the development of NEC. However, the genetic and molecular mechanisms underlying pyroptosis in NEC pathogenesis and sequelae remain unclear. Our study aimed to identify the pyroptosis-related cell populations and genes and explore potential therapeutic targets. Single-cell RNA sequencing (scRNA-seq) data were analyzed to identify the cell populations related to NEC and pyroptosis. Weighted gene correlation network analysis (WGCNA) of bulk RNA-seq was performed to identify gene modules associate with pyroptosis. Cell-cell communication was employed to investigate intercellular signaling networks. Gene Set Enrichment Analysis (GSEA) was conducted to compare the pathways enriched in the high and low TREM1-expressing subgroups. Immunofluorescence staining was performed to detect the TREM1

Indexed as

Enterocolitis, NecrotizingMacrophagesPyroptosisAnimalsAntigens, Differentiation, MyelomonocyticCD163 AntigenCell CommunicationGene Regulatory NetworksHumansMiceSequence Analysis, RNASingle-Cell AnalysisTriggering Receptor Expressed on Myeloid Cells-1Antigens, Differentiation, MyelomonocyticCD163 AntigenTREM1 protein, humanTriggering Receptor Expressed on Myeloid Cells-1bioinformaticsmacrophagenecrotizing enterocolitispyroptosisTREM1

Identifiers

PMID40362275
PMCPMC12071306

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.