Evidence map›Paper›PMID 40361189›Full record

ArticleRespiratory research2025

Ovarian Cancer G protein-coupled receptor-1 signaling bias dictates anti-contractile effect of benzodiazepines on airway smooth muscle.

Dominic R Villalba, Arun K Jannu, Elham Javed, Isha Dandekar, Ruping Wang, Deepak A Deshpande, Steven S An, Reynold A Panettieri, Dale D Tang, Raymond B Penn and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Respiratory research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dominic R VillalbaDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
Arun K JannuDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
Elham JavedDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
Isha DandekarRutgers University, New Brunswick, NJ, USA.
Ruping WangDepartment of Molecular and Cellular Physiology, Albany Medical College, Albany, NY, USA.
Deepak A DeshpandeDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
Steven S AnRutgers University, New Brunswick, NJ, USA.
Reynold A PanettieriRutgers University, New Brunswick, NJ, USA.
Dale D TangDepartment of Molecular and Cellular Physiology, Albany Medical College, Albany, NY, USA.
Raymond B PennDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
Ajay P NayakDepartment of Medicine, Center for Translational Medicine & Division of Pulmonary and Critical Care Medicine; Jane and Leonard Korman Lung Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA. ajay.nayak@jefferson.edu.

Funding

PLK1 Regulation of Airway Smooth MuscleR01HL130304 · NHLBI · ALBANY MEDICAL COLLEGE · PI TANG, DALE D. · 2016 to 2024
$3.5M
Mitochondrial translocator protein: a target for bronchodilationR01HL153602 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI NAYAK, AJAY · 2021 to 2025
$2.0M
NHLBI NIH HHS R01 HL130304NHLBI NIH HHS R01 HL153602NIH/NHLBI HL114471NIH/NHLBI HL153602
6 · The paper itself

Abstract

backgroundWe recently reported that the ovarian cancer G protein-coupled receptor-1 (OGR1) can be pharmacologically biased with specific benzodiazepines to couple with distinct heterotrimeric G proteins in human airway smooth muscle (ASM) cells. Lorazepam stimulated both G

methodsModels of histamine (His) -stimulated contraction included imaging of ex vivo human precision cut lung slices (hPCLS) and Magnetic Twisting Cytometry (MTC) analysis of human ASM cell stiffness. To explore mechanisms of regulation, we examined effects on myosin light chain (pMLC) phosphorylation and PKA activity in primary human ASM cultures, as well as actin cytoskeleton integrity as defined by changes in the ratio of F to G actin assessed by immunofluorescence.

resultsIn a dose-dependent manner, sulazepam relaxed His-contracted hPCLS and reduced baseline cell stiffness. Lorazepam did not relax His-contracted hPCLS, and only at a maximal dose (100 μM) did lorazepam relax baseline cell stiffness. The G

conclusionsThe G

Indexed as

BenzodiazepinesLungMuscle ContractionMuscle, SmoothOvarian NeoplasmsReceptors, G-Protein-CoupledCells, CulturedDose-Response Relationship, DrugFemaleHumansSignal TransductionBenzodiazepinesGPR68 protein, humanReceptors, G-Protein-CoupledAirway smooth muscleBenzodiazepinesGPCROGR1

Identifiers

PMID40361189
PMCPMC12076885

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.